Olfactory Regeneration with Nasally Administered Murine Adipose-Derived Stem Cells in Olfactory Epithelium Damaged Mice.
Olfactory Regeneration with Nasally Administered Murine Adipose-Derived Stem Cells in Olfactory Epithelium Damaged Mice.
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在这项研究中，我们的目的是确定是否鼻腔给药小鼠脂肪干细胞（ADSCs）可以支持嗅觉再生在体内。通过腹腔注射甲巯咪唑诱导8周龄C57BL/6J雄性小鼠的嗅上皮损伤。7天后，将从绿色荧光蛋白（GFP）转基因C57 BL/6小鼠获得的OriCell脂肪来源的间充质干细胞经鼻施用到这些小鼠的左鼻孔，并评估它们对丁酸的先天气味厌恶行为。与溶剂对照组动物相比，ADSC治疗后14 d，小鼠表现出明显的气味厌恶行为恢复，沿着，通过免疫组织化学染色评估，鼻中隔上皮中上部两侧的嗅觉标记蛋白（OMP）表达增加。ADSC培养上清中检测到神经生长因子（NGF），左侧鼻腔注射ADSC后24 h，小鼠鼻腔上皮中NGF表达增加，左侧鼻腔上皮表面可见GFP阳性细胞。本研究结果表明，鼻内给予分泌神经营养因子的ADSCs可刺激嗅上皮再生，从而促进体内气味厌恶行为的恢复。
In this study, we aimed to determine whether nasally administered murine adipose-derived stem cells (ADSCs) could support olfactory regeneration in vivo. Olfactory epithelium damage was induced in 8-week-old C57BL/6J male mice by intraperitoneal injection of methimazole. Seven days later, OriCell adipose-derived mesenchymal stem cells obtained from green fluorescent protein (GFP) transgenic C57BL/6 mice were nasally administered to the left nostril of these mice, and their innate odor aversion behavior to butyric acid was assessed. Mice showed significant recovery of odor aversion behavior, along with improved olfactory marker protein (OMP) expression on both sides of the upper-middle part of the nasal septal epithelium assessed by immunohistochemical staining 14 d after the treatment with ADSCs compared with vehicle control animals. Nerve growth factor (NGF) was detected in the ADSC culture supernatant, NGF was increased in the nasal epithelium of mice, and GFP-positive cells were observed on the surface of the left side nasal epithelium 24 h after left side nasal administration of ADSCs. The results of this study suggest that the regeneration of olfactory epithelium can be stimulated by nasally administered ADSCs secreting neurotrophic factors, thereby promoting the recovery of odor aversion behavior in vivo.
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影响因子：
1.7
作者：
Miwa, T;Uramoto, N;Morjizumi, T
通讯作者：
Morjizumi, T
影响因子：
1.3
作者：
Umehara, Tsuyoshi;Udagawa, Jun;Otani, Hiroki
通讯作者：
Otani, Hiroki
DOI：
10.1073/pnas.93.18.9858
发表时间：
1996-09-03
影响因子：
11.1
作者：
Buiakova, OI;Baker, H;Margolis, FL
通讯作者：
Margolis, FL
影响因子：
5.3
作者：
Aloe L;Rocco ML;Balzamino BO;Micera A
通讯作者：
Micera A
DOI：
10.1073/pnas.0911647107
发表时间：
2010-05-11
影响因子：
11.1
作者：
Kuroda, Yasumasa;Kitada, Masaaki;Dezawa, Mari
通讯作者：
Dezawa, Mari