Quantification of Growth of Campylobacter and Extended Spectrum β-Lactamase Producing Bacteria Sheds Light on Black Box of Enrichment Procedures.
Quantification of Growth of Campylobacter and Extended Spectrum β-Lactamase Producing Bacteria Sheds Light on Black Box of Enrichment Procedures.
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DOI：
10.3389/fmicb.2016.01430
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发表时间：
2016
影响因子：
5.2
通讯作者：
den Besten HM
中科院分区：
文献类型：
--
作者：
Hazeleger WC;Jacobs-Reitsma WF;den Besten HM
弯曲杆菌被公认为是全球细菌性食源性腹泻病的主要原因，并且通常在源自家禽、羊、猪和牛的肉类中发现。弯曲杆菌污染的有效监测取决于可靠检测方法的可用性。国际标准化组织检测弯曲杆菌属的方法。《食品中的微生物鉴定》（ISO 10272-1：2006）建议使用博尔顿肉汤（BB）作为选择性富集培养基，包括在37 ℃下进行4-6 h的预富集步骤，以在41.5 ℃下孵育2天之前复苏亚致死损伤的细胞。近年来，大量产超广谱β-内酰胺酶肠杆菌科细菌（ESBL菌）的存在已成为干扰弯曲杆菌分离的重要因素之一，导致假阴性检测。然而，弯曲杆菌及其竞争者的详细生长动力学仍不清楚，这些将为进一步改进弯曲杆菌的富集程序提供坚实的基础。其他富集肉汤，如普雷斯顿肉汤（PB）和BB加克拉维酸（BBc），已被建议抑制竞争性植物群。因此，这些不同的肉汤被用作增菌液，以分别测量空肠弯曲杆菌和ESBL细菌的几种菌株的生长动力学，共培养和鸡样品中的菌株。与ESBL细菌混合培养物中弯曲杆菌的最大细胞数量和生长速率通常显著低于单一培养物，表明ESBL细菌对弯曲杆菌的严重抑制，也是在自然污染的样品中。PB和BBc成功地减少了ESBL细菌，因此可能是可能被ESBL细菌污染的样品中作为增菌培养基的更好选择。对于冷强制降解和非强制降解细胞，不支持BB ISO程序中预富集步骤的有效性。因此，可能建议省略该步骤（在37°C下4-6小时）以获得不那么麻烦的方案。
Campylobacter is well recognized as the leading cause of bacterial foodborne diarrheal disease worldwide, and is routinely found in meat originating from poultry, sheep, pigs, and cattle. Effective monitoring of Campylobacter contamination is dependent on the availability of reliable detection methods. The method of the International Organization for Standardization for the detection of Campylobacter spp. in food (ISO 10272-1:2006) recommends the use of Bolton broth (BB) as selective enrichment medium, including a pre-enrichment step of 4–6 h at 37°C to revive sublethally damaged cells prior to incubation for 2 days at 41.5°C. Recently the presence of abundantly growing extended spectrum β-lactamase producing Enterobacteriaceae (ESBL bacteria) has become one of the most important factors that interfere with the isolation of Campylobacter, resulting in false-negative detection. However, detailed growth dynamics of Campylobacter and its competitors remain unclear, where these would provide a solid base for further improvement of the enrichment procedure for Campylobacter. Other enrichment broths, such as Preston broth (PB) and BB plus clavulanic acid (BBc) have been suggested to inhibit competitive flora. Therefore, these different broths were used as enrichments to measure the growth kinetics of several strains of Campylobacter jejuni and ESBL bacteria separately, in co-culture and of strains in chicken samples. The maximum cell numbers and often the growth rates of Campylobacter in mixed culture with ESBL bacteria were significantly lower than in single cultures, indicating severe suppression of Campylobacter by ESBL bacteria, also in naturally contaminated samples. PB and BBc successfully diminished ESBL bacteria and might therefore be a better choice as enrichment medium in possibly ESBL-bacteria contaminated samples. The efficacy of a pre-enrichment step in the BB ISO-procedure was not supported for cold-stressed and non-stressed cells. Therefore, omission of this step (4–6 h at 37°C) might be advised to obtain a less troublesome protocol.
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影响因子：
4.9
作者：
PAYNE, DJ;CRAMP, R;KNOWLES, DJC
通讯作者：
KNOWLES, DJC
DOI：
10.1039/9781847551450-00403
发表时间：
2012-01-01
期刊：
HANDBOOK OF CULTURE MEDIA FOR FOOD AND WATER MICROBIOLOGY, 3RD EDITION
影响因子：
--
作者：
Corry, Janet E. L.;Atabay, H. Ibrahim
通讯作者：
Atabay, H. Ibrahim
DOI：
10.1111/j.1365-2672.1986.tb04265.x
发表时间：
1986-08-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
HUMPHREY, TJ
通讯作者：
HUMPHREY, TJ
DOI：
10.1111/j.1365-2672.1993.tb03009.x
发表时间：
1993-02-01
期刊：
JOURNAL OF APPLIED BACTERIOLOGY
影响因子：
--
作者：
SCOTTER, SL;HUMPHREY, TJ;HENLEY, A
通讯作者：
HENLEY, A
影响因子：
5.2
作者：
Dierikx, Cindy;van der Goot, Jeanet;Mevius, Dik
通讯作者：
Mevius, Dik