Ecdysone receptor (EcR) is involved in the transcription of cell cycle genes in the silkworm.
Ecdysone receptor (EcR) is involved in the transcription of cell cycle genes in the silkworm.
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DOI：
10.3390/ijms16023335
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发表时间：
2015-02-03
影响因子：
5.6
通讯作者：
Cheng D
中科院分区：
文献类型：
--
作者：
Qian W;Kang L;Zhang T;Meng M;Wang Y;Li Z;Xia Q;Cheng D
蜕皮激素受体(ECR)介导的蜕皮激素信号通路有助于调节昆虫发育过程中参与各种过程的基因的转录。在本工作中，我们检测了ECR基因在家蚕卵巢来源的BmN4细胞中的表达，发现ECR RNAi导致了细胞形态的改变，表明ECR可能协调细胞周期的进程。ECR RNAi和ECR过表达分析表明，在培养的BmN4细胞中，ECR分别促进和抑制控制细胞周期进程的两个基因E2F-1和CycE的转录。进一步研究表明，蜕皮激素作用于BmN4细胞，不仅改变了ECR过表达条件下这两种细胞周期基因的转录水平，而且使细胞周期停滞于G2/M期。体内分析证实，蜕皮激素处理后，家蚕幼虫丝腺中E2F-1的表达增加，这与其在BmN4细胞中对蜕皮激素的反应相同。然而，蜕皮激素也促进丝腺中CycE的转录，这与在BmN4细胞中的观察结果相反。这些结果为理解ECR介导的蜕皮激素信号在细胞周期调控中的作用提供了新的见解。
EcR (ecdysone receptor)-mediated ecdysone signaling pathway contributes to regulate the transcription of genes involved in various processes during insect development. In this work, we detected the expression of EcR gene in silkworm ovary-derived BmN4 cells and found that EcR RNAi result in an alteration of cell shape, indicating that EcR may orchestrate cell cycle progression. EcR RNAi and EcR overexpression analysis revealed that in the cultured BmN4 cells, EcR respectively promoted and suppressed the transcription of E2F-1 and CycE, two genes controlling cell cycle progression. Further examination demonstrated that ecdysone application in BmN4 cells not only changed the transcription of these two cell cycle genes like that under EcR overexpression, but also induced cell cycle arrest at G2/M phase. In vivo analysis confirmed that E2F-1 expression was elevated in silk gland of silkworm larvae after ecdysone application, which is same as its response to ecdysone in BmN4 cells. However, ecdysone also promotes CycE transcription in silk gland, and this is converse with the observation in BmN4 cells. These results provide new insights into understanding the roles of EcR-mediated ecdysone signaling in the regulation of cell cycle.
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影响因子：
2.3
作者：
Cranna N;Quinn L
通讯作者：
Quinn L
影响因子：
3.7
作者：
Giraudo M;Califano J;Hilliou F;Tran T;Taquet N;Feyereisen R;Le Goff G
通讯作者：
Le Goff G
影响因子：
3.7
作者：
Li Z;Cheng D;Mon H;Tatsuke T;Zhu L;Xu J;Lee JM;Xia Q;Kusakabe T
通讯作者：
Kusakabe T
影响因子：
64.5
作者：
KOELLE, MR;TALBOT, WS;HOGNESS, DS
通讯作者：
HOGNESS, DS
DOI：
10.1073/pnas.94.22.12024
发表时间：
1997-10-28
影响因子：
11.1
作者：
Carney, GE;Wade, AA;Bender, M
通讯作者：
Bender, M