Autophagy Regulation Using Multimodal Chlorin e6-Loaded Polysilsesquioxane Nanoparticles to Improve Photodynamic Therapy.
Autophagy Regulation Using Multimodal Chlorin e6-Loaded Polysilsesquioxane Nanoparticles to Improve Photodynamic Therapy.
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DOI：
10.3390/pharmaceutics15051548
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发表时间：
2023-05-20
期刊：
影响因子：
5.4
通讯作者：
Vivero-Escoto JL
中科院分区：
文献类型：
--
作者：
Vadarevu H;Sorinolu AJ;Munir M;Vivero-Escoto JL
光动力疗法(PDT)是一种依赖于活性氧(ROS)生成的抗癌非侵入性治疗技术。不幸的是，光动力疗法仍然有许多局限性，包括癌细胞对ROS的细胞毒作用产生的耐药性。自噬是一种应激反应机制，已被报道为减少PDT后细胞死亡的一种细胞途径。最近的研究表明，光动力疗法与其他疗法相结合可以消除抗癌耐药性。然而，联合治疗通常受到药物药代动力学差异的挑战。纳米材料是高效共传递两种或更多治疗剂的极佳递送系统。在这项工作中，我们报告了使用聚倍半硅氧烷(PSilQ)纳米颗粒共传递氯-e6(Ce6)和用于早期或晚期自噬的自噬抑制剂。我们的结果来自活性氧物种(ROS)的产生以及细胞凋亡和自噬通量的分析，结果表明，由组合方法介导的自噬通量的减少增加了Ce6-PSilQ纳米颗粒的光疗效率。我们预计，将多模式CE6-PSilQ材料用作抗癌联合递送系统的前景良好的结果，将为其未来与其他临床相关组合的应用铺平道路。
Photodynamic therapy (PDT) is a promising anticancer noninvasive technique that relies on the generation of reactive oxygen species (ROS). Unfortunately, PDT still has many limitations, including the resistance developed by cancer cells to the cytotoxic effect of ROS. Autophagy, which is a stress response mechanism, has been reported as a cellular pathway that reduces cell death following PDT. Recent studies have demonstrated that PDT in combination with other therapies can eliminate anticancer resistance. However, combination therapy is usually challenged by the differences in the pharmacokinetics of the drugs. Nanomaterials are excellent delivery systems for the efficient codelivery of two or more therapeutic agents. In this work, we report on the use of polysilsesquioxane (PSilQ) nanoparticles for the codelivery of chlorin-e6 (Ce6) and an autophagy inhibitor for early- or late-stage autophagy. Our results, obtained from a reactive oxygen species (ROS) generation assay and apoptosis and autophagy flux analyses, demonstrate that the reduced autophagy flux mediated by the combination approach afforded an increase in the phototherapeutic efficacy of Ce6-PSilQ nanoparticles. We envision that the promising results in the use of multimodal Ce6-PSilQ material as a codelivery system against cancer pave the way for its future application with other clinically relevant combinations.
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影响因子：
5.6
作者：
Bacellar IO;Tsubone TM;Pavani C;Baptista MS
通讯作者：
Baptista MS
影响因子：
5.6
作者：
Ichimiya T;Yamakawa T;Hirano T;Yokoyama Y;Hayashi Y;Hirayama D;Wagatsuma K;Itoi T;Nakase H
通讯作者：
Nakase H
影响因子：
6
作者：
Inguscio V;Panzarini E;Dini L
通讯作者：
Dini L
影响因子：
16
作者：
Kroemer G;Mariño G;Levine B
通讯作者：
Levine B
DOI：
10.1007/978-1-60761-411-1_4
发表时间：
2010-01-01
期刊：
ADVANCED PROTOCOLS IN OXIDATIVE STRESS II
影响因子：
--
作者：
Eruslanov, Evgeniy;Kusmartsev, Sergei
通讯作者：
Kusmartsev, Sergei