LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat
LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat
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LncRNA-599554海绵miR-15a-5p通过正向调节绒山羊中Wnt3a的表达来贡献真皮乳头细胞的诱导能力
DOI：
10.1016/j.ejbt.2020.03.002
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发表时间：
2020-05
影响因子：
2.7
通讯作者：
Bai Wen L.
中科院分区：
文献类型：
--
作者：
Yin Rong H.;Wang Yan R.;Zhao Su J.;Yin Rong L.;Bai Man;Wang Ze Y.;Zhu Yu B.;Cong Yu Y.;Liu Hai Y.;Bai Wen L.
研究背景长链非编码RNA（lncRNA）作为转录后调节因子，在绒山羊毛乳头细胞（dermal papilla cells，DPC）的诱导特性中发挥重要作用。以前，lncRNA-599554被确定在次级毛囊（SHF）的绒山羊，但其功能的意义是unknow.ResultsIn目前的调查，我们验证了lncRNA-599554有显着较高的表达在生长期毛乳头的绒山羊SHF比在休止期。本研究通过检测内皮素-1（ET-1）、干细胞因子（SCF）、Versican、碱性磷酸酶（ALP）、Lef 1和Ptc-1等几个典型指示基因的表达变化，发现lncRNA-599554参与了绒山羊毛乳头细胞的诱导。基于RNA pull-down分析，我们验证了lncRNA-599554与chi-miR-15 a-5 p直接相互作用。此外，我们发现lncRNA-599554正调控DPC中Wnt 3a的表达，但似乎不涉及其对启动子甲基化的调节。结论lncRNA-599554对绒山羊毛乳头细胞的诱导作用可能是通过吸收chi-miR-15 b-5 p来促进Wnt 3a的表达。本研究的结果为lncRNA-599554在绒山羊SHF再生中的作用机制提供了新的见解，沿着绒纤维的形成和生长。如何引用：Yin RH，Wang YR，Zhao SJ，et al. LncRNA-599554 sponges miR-15 a-5 p to contribute inductive ability of dermal papilla cells through positive regulation of Wnt 3a in cashmere goat. 45.第四十五章：一夜情https://doi.org/10.1016/j.ejbt.2020.03.002
BackgroundLong non-coding RNAs (lncRNAs), as post-transcriptional regulators, were thought to function in the inductive property of dermal papilla cells (DPCs) in cashmere goat. Previously, lncRNA-599554 was identified in secondary hair follicle (SHF) of cashmere goat, but its functional significance is unknown.ResultsIn the present investigation, we verified that lncRNA-599554 had significantly higher expression at the anagen dermal papilla of cashmere goat SHF than that at telogen. Based on overexpression and knockdown techniques, we found that lncRNA-599554 contributes the inductive property of DPCs of cashmere goat, which was assessed by detecting the changes in the expression of several typical indictor genes in DPCs including ET-1, SCF, Versican, ALP, Lef1 and Ptc-1. Based on RNA pull-down assay, we verified that lncRNA-599554 directly interacted with chi-miR-15a-5p. Also, we showed that lncRNA-599554 positively regulated the Wnt3a expression in DPCs but which did not appear to involve its modulating of promoter methylation. Based on the use of Dual-luciferase reporter assays, our data indicated that lncRNA-599554 regulated the Wnt3a expression through chi-miR-15a-5p-mediated post-transcriptional level.ConclusionsWe showed that lncRNA-599554 contributes the inductive property of DPCs in cashmere goat which might be achieved through sponging chi-miR-15b-5p to promote the Wnt3a expression. The results from the present investigation provided a novel insight into the functional mechanism of lncRNA-599554 in the SHF regeneration of cashmere goat along with the formation and growth of cashmere fiber.How to cite:Yin RH, Wang YR, Zhao SJ, et al. LncRNA-599554 sponges miR-15a-5p to contribute inductive ability of dermal papilla cells through positive regulation of the expression of Wnt3a in cashmere goat. Electron J Biotechnol 2020;45. https://doi.org/10.1016/j.ejbt.2020.03.002
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DOI：
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发表时间：
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期刊：
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影响因子：
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影响因子：
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影响因子：
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