Biogenic synthesis of Zinc oxide nanostructures from Nigella sativa seed: Prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm.
Biogenic synthesis of Zinc oxide nanostructures from Nigella sativa seed: Prospective role as food packaging material inhibiting broad-spectrum quorum sensing and biofilm.
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DOI：
10.1038/srep36761
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发表时间：
2016-12-05
影响因子：
4.6
通讯作者：
Aliev G
中科院分区：
文献类型：
--
作者：
Al-Shabib NA;Husain FM;Ahmed F;Khan RA;Ahmad I;Alsharaeh E;Khan MS;Hussain A;Rehman MT;Yusuf M;Hassan I;Khan JM;Ashraf GM;Alsalme A;Al-Ajmi MF;Tarasov VV;Aliev G
细菌对食品的腐败是由密度依赖的通讯系统--群体感应系统（QS）调控的。QS控制许多食品病原体的生物膜形成，而在食品表面形成的生物膜充当细菌污染的载体，导致食品腐败和健康危害。抑制或干扰细菌QS和生物膜的试剂作为一类新的下一代食品防腐剂/包装材料越来越重要。在本研究中，锌纳米结构的合成使用黑种草种子提取物（NS-ZnNPs）。通过UV-vis、XRD、FTIR和TEM等测试手段对合成的纳米结构进行了表征，结果表明纳米结构为六方纤锌矿结构，尺寸约为24 nm。NS-ZnNPs在C. violaceum和铜绿假单胞菌生物传感器菌株。合成的纳米结构抑制了C.而PAO 1则显著提高了CVO 26（紫霉素）和弹性蛋白酶、蛋白酶、绿脓菌素和藻酸盐的产量。亚抑菌浓度的NS-ZnNPs抑制了四种食品病原菌的生物膜形成，即C。violaceum 12472、PAO 1、L.单核细胞增生E.杆菌此外，NS-ZnNPs被发现有效地抑制四种病原体的预形成的成熟生物膜。因此，生物源性氧化锌纳米颗粒对QS和生物膜的广谱抑制，并且设想这些无毒的生物活性纳米结构可以用作食品包装材料和/或食品防腐剂。
Bacterial spoilage of food products is regulated by density dependent communication system called quorum sensing (QS). QS control biofilm formation in numerous food pathogens and Biofilms formed on food surfaces act as carriers of bacterial contamination leading to spoilage of food and health hazards. Agents inhibiting or interfering with bacterial QS and biofilm are gaining importance as a novel class of next-generation food preservatives/packaging material. In the present study, Zinc nanostructures were synthesised using Nigella sativa seed extract (NS-ZnNPs). Synthesized nanostructures were characterized hexagonal wurtzite structure of size ~24 nm by UV-visible, XRD, FTIR and TEM. NS-ZnNPs demonstrated broad-spectrum QS inhibition in C. violaceum and P. aeruginosa biosensor strains. Synthesized nanostructures inhibited QS regulated functions of C. violaceum CVO26 (violacein) and elastase, protease, pyocyanin and alginate production in PAO1 significantly. NS-ZnNPs at sub-inhibitory concentrations inhibited the biofilm formation of four-food pathogens viz. C. violaceum 12472, PAO1, L. monocytogenes, E. coli. Moreover, NS-ZnNPs was found effective in inhibiting pre-formed mature biofilms of the four pathogens. Therefore, the broad-spectrum inhibition of QS and biofilm by biogenic Zinc oxide nanoparticles and it is envisaged that these nontoxic bioactive nanostructures can be used as food packaging material and/or as food preservative.
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影响因子：
3.9
作者：
Glisic, Biljana D.;Senerovic, Lidija;Nikodinovic-Runic, Jasmina
通讯作者：
Nikodinovic-Runic, Jasmina
影响因子：
15.9
作者：
Hentzer, M;Givskov, M
通讯作者：
Givskov, M
影响因子：
6.8
作者：
Amooaghaie, Rayhaneh;Saeri, Mohammad Reza;Azizi, Morteza
通讯作者：
Azizi, Morteza
影响因子：
3.7
作者：
Gopu V;Meena CK;Shetty PH
通讯作者：
Shetty PH
影响因子：
4.1
作者：
Horie, Masanori;Nishio, Keiko;Yoshida, Yasukazu
通讯作者：
Yoshida, Yasukazu