Damage-free peripheral nerve stimulation by 12-ns pulsed electric field.
Damage-free peripheral nerve stimulation by 12-ns pulsed electric field.
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DOI：
10.1038/s41598-017-10282-5
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发表时间：
2017-09-05
影响因子：
4.6
通讯作者：
Pakhomov AG
中科院分区：
文献类型：
--
作者：
Casciola M;Xiao S;Pakhomov AG
现代技术使纳秒脉冲电场（nsPEF）的深层组织聚焦成为非侵入性神经和肌肉刺激。然而，目前尚不清楚短于电压门控钠通道（VGSC）激活时间的PEF是否会引起动作电位（APs）。一种可能的情况是膜完整性的丧失（电穿孔）和由此产生的去极化作为中间步骤。我们首次报道了周围神经的兴奋可以在没有电穿孔的情况下通过12ns PEF完成。4.1 ~ 11 kV (3.3 ~ 8.8 kV/cm) 12 ns刺激诱发的ap与常规刺激（100 ~ 250 μs, 1 ~ 5 V, 103 ~ 515 V/m）相似，但对较快神经纤维的选择性更高。神经持续以12 ns PEF和常规脉冲交替进行50 Hz或100 Hz 1分钟的强直刺激。这种破伤风引起了轻微的AP下降，两种刺激的程度相似。神经不应性不受影响。即使在成千上万的12ns刺激中也没有累积损伤，并且与传统刺激相似，证明nsPEF激活VGSC没有神经膜损伤。
Modern technologies enable deep tissue focusing of nanosecond pulsed electric field (nsPEF) for non-invasive nerve and muscle stimulation. However, it is not known if PEF orders of magnitude shorter than the activation time of voltage-gated sodium channels (VGSC) would evoke action potentials (APs). One plausible scenario requires the loss of membrane integrity (electroporation) and resulting depolarization as an intermediate step. We report, for the first time, that the excitation of a peripheral nerve can be accomplished by 12-ns PEF without electroporation. 12-ns stimuli at 4.1–11 kV (3.3–8.8 kV/cm) evoked APs similarly to conventional stimuli (100–250 μs, 1–5 V, 103–515 V/m), except for having higher selectivity for the faster nerve fibers. Nerves sustained repeated tetanic stimulations (50 Hz or 100 Hz for 1 min) alternately by 12-ns PEF and by conventional pulses. Such tetani caused a modest AP decrease, to a similar extent for both types of stimuli. Nerve refractory properties were not affected. The lack of cumulative damages even from tens of thousands of 12-ns stimuli and the similarities with the conventional stimulation prove VGSC activation by nsPEF without nerve membrane damage.
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影响因子：
5
作者：
Ibey, Bennett L.;Mixon, Dustin G.;Payne, Jason A.;Bowman, Angela;Sickendick, Karl;Wilmink, Gerald J.;Roach, W. Patrick;Pakhomov, Andrei G.
通讯作者：
Pakhomov, Andrei G.
DOI：
10.1016/j.bbrc.2013.12.004
发表时间：
2014-01-10
影响因子：
3.1
作者：
Ibey, Bennett L.;Ullery, Jody C.;Pakhomova, Olga N.;Roth, Caleb C.;Semenov, Iurii;Beier, Hope T.;Tarango, Melissa;Xiao, Shu;Schoenbach, Karl H.;Pakhomov, Andrei G.
通讯作者：
Pakhomov, Andrei G.
影响因子：
4.6
作者：
de Menorval MA;Andre FM;Silve A;Dalmay C;Français O;Le Pioufle B;Mir LM
通讯作者：
Mir LM
影响因子：
1.9
作者：
Pakhomov, Andrei G.;Kolb, Juergen F.;Schoenbach, Karl H.
通讯作者：
Schoenbach, Karl H.
影响因子：
5.1
作者：
Schlenstedt, C.;Shalash, A.;Deuschl, G.
通讯作者：
Deuschl, G.