Spinal cord injury causes sustained disruption of the blood-testis barrier in the rat.
Spinal cord injury causes sustained disruption of the blood-testis barrier in the rat.
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DOI：
10.1371/journal.pone.0016456
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发表时间：
2011-01-26
期刊：
影响因子：
3.7
通讯作者：
Grill RJ
中科院分区：
文献类型：
--
作者：
Dulin JN;Moore ML;Gates KW;Queen JH;Grill RJ
外伤性脊髓损伤（SCI）后男性不育的发生率很高。这些患者的精液质量通常较差，但导致这种情况的病理生理机制尚不清楚。先前尚未检查 SCI 后血睾屏障 (BTB) 的完整性。本研究的目的是表征脊髓挫伤对大鼠 BTB 的影响。 63 只成年雄性 Sprague Dawley 大鼠接受 SCI (n = 28)、仅椎板切除术 (n = 7) 或作为未受伤、年龄匹配的对照 (n = 28)。使用动态对比增强磁共振成像 (DCE-MRI)，在 SCI 后 72 小时或 10 个月评估 BTB 对血管造影剂钆喷酸二葡胺 (Gd) 的渗透性。 DCE-MRI 数据显示，在 SCI 后 72 小时和 10 个月，BTB 对 Gd 的渗透性均大于对照组。睾丸组织的组织学评估显示，与年龄匹配的假手术组和未受伤对照组相比，SCI 后 72 小时和 10 个月 BTB 对免疫球蛋白 G 的通透性增加。评估生精上皮内的紧密连接完整性； SCI 后 72 小时，观察到紧密连接蛋白 occludin 的表达下降。还检查了睾丸是否存在炎症。在睾丸组织中检测到促炎细胞因子白介素-1β的高表达。在急性和慢性 SCI 组的生精上皮内也检测到 CD68+ 免疫细胞浸润和肥大细胞，但在对照组中未检测到。此外，SCI 后 72 小时观察到广泛的生殖细胞凋亡。基于这些结果，我们得出结论，大鼠损伤后 72 小时内，SCI 后 BTB 完整性就会受损，并伴有睾丸内的大量免疫反应。此外，我们的结果表明，BTB 仍然受到损害，睾丸免疫细胞浸润在初次损伤后持续数月。
There is a high incidence of infertility in males following traumatic spinal cord injury (SCI). Quality of semen is frequently poor in these patients, but the pathophysiological mechanism(s) causing this are not known. Blood-testis barrier (BTB) integrity following SCI has not previously been examined. The objective of this study was to characterize the effects of spinal contusion injury on the BTB in the rat. 63 adult, male Sprague Dawley rats received SCI (n = 28), laminectomy only (n = 7) or served as uninjured, age-matched controls (n = 28). Using dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI), BTB permeability to the vascular contrast agent gadopentate dimeglumine (Gd) was assessed at either 72 hours-, or 10 months post-SCI. DCE-MRI data revealed that BTB permeability to Gd was greater than controls at both 72 h and 10 mo post-SCI. Histological evaluation of testis tissue showed increased BTB permeability to immunoglobulin G at both 72 hours- and 10 months post-SCI, compared to age-matched sham-operated and uninjured controls. Tight junctional integrity within the seminiferous epithelium was assessed; at 72 hours post-SCI, decreased expression of the tight junction protein occludin was observed. Presence of inflammation in the testes was also examined. High expression of the proinflammatory cytokine interleukin-1 beta was detected in testis tissue. CD68+ immune cell infiltrate and mast cells were also detected within the seminiferous epithelium of both acute and chronic SCI groups but not in controls. In addition, extensive germ cell apoptosis was observed at 72 h post-SCI. Based on these results, we conclude that SCI is followed by compromised BTB integrity by as early as 72 hours post-injury in rats and is accompanied by a substantial immune response within the testis. Furthermore, our results indicate that the BTB remains compromised and testis immune cell infiltration persists for months after the initial injury.
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影响因子：
3.6
作者：
Gerdprasert, O;O'Bryan, MK;Hedger, MP
通讯作者：
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影响因子：
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作者：
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