Age Dependence of Systemic Bone Loss and Recovery Following Femur Fracture in Mice.
Age Dependence of Systemic Bone Loss and Recovery Following Femur Fracture in Mice.
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DOI：
10.1002/jbmr.3579
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发表时间：
2019-01
期刊：
影响因子：
--
通讯作者：
Christiansen BA
中科院分区：
文献类型：
--
作者：
Emami AJ;Toupadakis CA;Telek SM;Fyhrie DP;Yellowley CE;Christiansen BA
未来骨折风险最可靠的预测因素是以前的任何类型的骨折。这种骨折风险增加的病因尚不完全清楚，但骨折可能引发全身性骨丢失，导致所有骨骼部位的骨折风险增加。在这项研究中，我们调查了年轻（3个月大）和中年（12个月大）小鼠股骨骨折后的全身骨丢失和恢复。使用受控冲击产生横向股骨骨折，并在骨折后多个时间点定量全身骨矿物质密度（BMD）、小梁和皮质微观结构、骨机械性能、骨形成和吸收率、小鼠自主运动和全身炎症。我们发现，骨折导致年轻和中年小鼠骨折后2周全身BMD下降;年轻小鼠的骨丢失在6周后恢复，但中年小鼠没有。同样，骨折小鼠L5椎体的骨小梁体积分数（BV/TV）在骨折后2周相对于对照小鼠显著降低（年轻小鼠为− 11%，中年小鼠为− 18%）;骨折后6周没有观察到显著差异。在骨折后3天，我们观察到骨折小鼠的血清白细胞介素-6水平显著增加，与对照小鼠相比，骨折小鼠的自主运动显著减少，中年小鼠的变化比年轻小鼠大得多。在这个时间点，我们还观察到与对照小鼠相比，骨折小鼠的L5椎体骨小梁上的破骨细胞数量增加。这些数据表明，年轻和中年小鼠骨折后都会发生全身性骨丢失，并且这种骨丢失的恢复可能随年龄而变化。这种全身反应可能导致骨折后骨折风险增加;这些数据可能为骨折的临床治疗提供信息，以改善长期骨骼健康。
The most reliable predictor of future fracture risk is a previous fracture of any kind. The etiology of this increased fracture risk is not fully known, but it is possible that fracture initiates systemic bone loss leading to greater fracture risk at all skeletal sites. In this study we investigated systemic bone loss and recovery following femoral fracture in young (3 month old) and middle-aged (12 month old) mice. Transverse femur fractures were created using a controlled impact, and whole-body bone mineral density (BMD), trabecular and cortical microstructure, bone mechanical properties, bone formation and resorption rates, mouse voluntary movement, and systemic inflammation were quantified at multiple time points post-fracture. We found that fracture led to decreased whole-body BMD in both young and middle-aged mice 2 weeks post-fracture; this bone loss was recovered by 6 weeks in young, but not middle-aged mice. Similarly, trabecular bone volume fraction (BV/TV) of the L5 vertebral body was significantly reduced in fractured mice relative to control mice 2 weeks post-fracture (−11% for young mice, −18% for middle-aged mice); no significant differences were observed 6 weeks post-fracture. At 3 days post-fracture we observed significant increases in serum levels of interleukin-6 and significant decreases in voluntary movement in fractured mice compared to control mice, with considerably greater changes in middle-aged mice than in young mice. At this time point we also observed increased osteoclast number on L5 vertebral body trabecular bone of fractured mice compared to control mice. These data show that systemic bone loss occurs after fracture in both young and middle-aged mice, and recovery from this bone loss may vary with age. This systemic response could contribute to increased future fracture risk following fracture; these data may inform clinical treatment of fractures with respect to improving long-term skeletal health.
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影响因子：
4.6
作者：
Fischer V;Haffner-Luntzer M;Prystaz K;Vom Scheidt A;Busse B;Schinke T;Amling M;Ignatius A
通讯作者：
Ignatius A
影响因子：
7.5
作者：
Gibon E;Lu L;Goodman SB
通讯作者：
Goodman SB
影响因子：
3.8
作者：
Li, Yan;Bakesjo, Carl-Magnus;Lindgren, Urban
通讯作者：
Lindgren, Urban
DOI：
10.2106/00004623-200310000-00011
发表时间：
2003-10-01
影响因子：
5.3
作者：
Haentjens, P;Autier, P;Boonen, S
通讯作者：
Boonen, S
影响因子：
2.4
作者：
Hou, FJ;Lang, SM;Fyhrie, DP
通讯作者：
Fyhrie, DP