Autoinducer2 affects trimethoprim-sulfamethoxazole susceptibility in avian pathogenic Escherichia coli dependent on the folate synthesis-associate pathway.
Autoinducer2 affects trimethoprim-sulfamethoxazole susceptibility in avian pathogenic Escherichia coli dependent on the folate synthesis-associate pathway.
复制标题
Autoinducer2 影响禽类致病性大肠杆菌中甲氧苄氨嘧啶-磺胺甲恶唑的敏感性，依赖于叶酸合成相关途径。
DOI：
10.1002/mbo3.582
复制
发表时间：
2018-08
期刊：
影响因子：
3.4
通讯作者：
Xue T
中科院分区：
文献类型：
--
作者：
Yu L;Li W;Zhang M;Cui Y;Chen X;Ni J;Yu L;Shang F;Xue T
禽致病性大肠杆菌(APEC)可引起鸡、鸭、鹅、鸽等禽类的气囊炎、多浆膜炎、败血症等主要肠外疾病，给养禽业造成巨大的经济损失。自动诱导因子2(AI-2)群体感应系统广泛存在于多种革兰氏阴性和革兰氏阳性细菌中，并被认为参与了物种间的交流。在临床APEC菌株中，AI-2是否影响抗生素相关基因的表达尚未见报道。在这项研究中，我们报道了外源AI-2通过叶酸合成依赖的途径而不是LsrR依赖的方式增加APEC菌株对甲氧普林-磺胺甲恶唑(SXT)的敏感性。我们的结果进一步解释了外源AI-2可以下调叶酸合成酶编码基因fo1和folC以及与叶酸合成相关的基因LuxS、mete和meth的转录。凝胶漂移分析证实，AI-2受体LsrR不与FOLA和FolC的启动子结合，提示外源AI-2可能通过反馈抑制作用影响叶酸代谢，但不是通过依赖LsrR的途径。本研究可能为寻找预防禽类大肠杆菌病的潜在药物靶点和治疗禽类大肠杆菌病的辅助抗生素提供进一步的信息。
Avian pathogenic Escherichia coli (APEC) causes airsacculitis, polyserositis, septicemia, and other mainly extraintestinal diseases in chickens, ducks, geese, pigeons, and other avian species, and is responsible for great economic losses in the avian industry. The autoinducer 2 (AI‐2) quorum sensing system is widely present in many species of gram‐negative and gram‐positive bacteria and has been proposed to be involved in interspecies communication. In clinical APEC strains, whether or not AI‐2 affects the expression of antibiotic‐related genes has not been reported. In this study, we have reported that exogenous AI‐2 increase the susceptibility of APEC strains to trimethoprim‐sulfamethoxazole (SXT) in a folate synthesis‐dependent pathway but not in the LsrR‐dependent manner. Our results further explained that exogenous AI‐2 can down regulate the transcription of the folate synthetase encoding genes folA and folC, and the folate synthesis‐related genes luxS, metE, and metH. Gel shift assays confirmed that LsrR, the AI‐2 receptor, did not bind to the promoters of folA and folC, suggesting that exogenous AI‐2 might influence folate metabolism by a feedback inhibition effect but not in the LsrR‐dependent pathway. This study might provide further information in the search for potential drug targets for prophylaxis of avian colibacillosis and for auxiliary antibiotics in the treatment of avian colibacillosis.
登录
查看更多内容
影响因子：
4.4
作者：
QUERALT, J;CASTELLS, I
通讯作者：
CASTELLS, I
影响因子：
1.3
作者：
Boothe, Dawn;Smaha, Tim;Hatchcock, Terri
通讯作者：
Hatchcock, Terri
影响因子：
9.4
作者：
Schouler, Catherine;Schaeffer, Brigitte;Moulin-Schouleur, Maryvonne
通讯作者：
Moulin-Schouleur, Maryvonne
影响因子：
9.4
作者：
Blanco, JE;Blanco, M;Blanco, J
通讯作者：
Blanco, J
DOI：
10.2460/javma.2002.221.411
发表时间：
2002-08-01
期刊：
JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
影响因子：
--
作者：
Altekruse, SF;Elvinger, F;Sriranganathan, N
通讯作者：
Sriranganathan, N