Expression of vesicular glutamate transporters in sensory and autonomic neurons innervating the mouse bladder.
Expression of vesicular glutamate transporters in sensory and autonomic neurons innervating the mouse bladder.
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DOI：
10.1016/j.juro.2012.11.046
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发表时间：
2013-06
影响因子：
6.6
通讯作者：
Gebhart, G. F.
中科院分区：
文献类型：
--
作者：
Brumovsky, Pablo R.;Seal, Rebecca P.;Lundgren, Kerstin H.;Seroogy, Kim B.;Watanabe, Masahiko;Gebhart, G. F.
VGLUT是将谷氨酸加载到突触小泡中所必需的物质，存在于各种神经系统中。然而，据我们所知，VGLUT在支配膀胱的神经元中的表达尚未被分析。我们研究了小鼠膀胱神经元的VGLUT1、VGLUT2和VGLUT3。采用免疫组织化学方法观察BALB/c小鼠膀胱壁注射快蓝后，VGLUT1、VGLUT2和降钙素基因相关肽在逆行标记的初级传入神经和自主神经中的表达。为了研究VGLUT3，我们追踪了转基因小鼠的膀胱，其中VGLUT3是通过增强的绿色荧光蛋白检测来鉴定的。大多数膀胱背根神经节神经元表达VGLUT2。少数神经元也表达VGLUT1或VGLUT3。仅VGLUT2与降钙素基因相关肽共表达。盆神经节内偶见VGLUT2免疫反应阳性神经元。VGLUT2免疫反应神经分布于膀胱穹隆、三角区和尿路。VGLUT1免疫反应神经散在分布。我们提供了我们所知的关于VGLUT在支配小鼠膀胱的感觉神经元和自主神经元中表达的新数据。VGLUT2和降钙素基因相关肽在感觉神经元中的频繁联系表明，谷氨酸能和肽能神经传递之间存在相互作用，可能会影响膀胱常见的感觉，如不适和疼痛。
VGLUTs, which are essential for loading glutamate into synaptic vesicles, are present in various neuronal systems. However, to our knowledge the expression of VGLUTs in neurons innervating the bladder has not yet been analyzed. We studied VGLUT1, VGLUT2 and VGLUT3 in mouse bladder neurons. We analyzed the expression of VGLUT1, VGLUT2 and calcitonin gene-related peptide by immunohistochemistry in the retrograde labeled primary afferent and autonomic neurons of BALB/c mice after injecting fast blue in the bladder wall. To study VGLUT3 we traced the bladder of transgenic mice, in which VGLUT3 is identified by enhanced green fluorescent protein detection. Most bladder dorsal root ganglion neurons expressed VGLUT2. A smaller percentage of neurons also expressed VGLUT1 or VGLUT3. Co-expression with calcitonin gene-related peptide was only observed for VGLUT2. Occasional VGLUT2 immunoreactive neurons were seen in the major pelvic ganglia. Abundant VGLUT2 immunoreactive nerves were detected in the bladder dome and trigone, and the urethra. VGLUT1 immunoreactive nerves were discretely present. We present what are to our knowledge novel data on VGLUT expression in sensory and autonomic neurons innervating the mouse bladder. The frequent association of VGLUT2 and calcitonin gene-related peptide in sensory neurons suggests interactions between glutamatergic and peptidergic neurotransmissions, potentially influencing commonly perceived sensations in the bladder, such as discomfort and pain.
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影响因子：
--
作者：
Robinson DR;Gebhart GF
通讯作者：
Gebhart GF
影响因子：
3.3
作者：
Crock, Lara W.;Stemler, Kristina M.;Gereau, Robert W.
通讯作者：
Gereau, Robert W.
影响因子：
2.5
作者：
Boulland, JL;Qureshi, T;Chaudhry, FA
通讯作者：
Chaudhry, FA
影响因子：
5.5
作者：
Brierley, SM;Carter, R;Blackshaw, LA
通讯作者：
Blackshaw, LA
影响因子：
1.4
作者：
Liguz-Lecznar, Monika;Skangiel-Kramska, Jolanta
通讯作者：
Skangiel-Kramska, Jolanta