Motor Learning Consolidates Arc-Expressing Neuronal Ensembles in Secondary Motor Cortex.
Motor Learning Consolidates Arc-Expressing Neuronal Ensembles in Secondary Motor Cortex.
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DOI：
10.1016/j.neuron.2015.05.022
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发表时间：
2015-06-17
期刊：
影响因子：
16.2
通讯作者：
Wang KH
中科院分区：
文献类型：
--
作者：
Cao VY;Ye Y;Mastwal S;Ren M;Coon M;Liu Q;Costa RM;Wang KH
运动行为在运动皮层中招募任务特异性神经元集合，这些集合在随后的学习中得到巩固。然而，人们对能够识别参与神经元并预测巩固过程结果的分子知之甚少。使用小鼠旋转杆学习任务，我们表明，损伤或失活的次级运动（M2）皮层破坏学习熟练的动作。我们跟踪内源性启动子活性的神经元活性调节基因弧在个别M2神经元在旋转学习过程中，在体内双光子成像的敲入报告。我们发现，任务训练最初招募ARC启动子激活的神经元，然后将它们整合到一个特定的合奏表现出持续的再激活的ARC启动子。神经元的初始Arc启动子激活的强度预测其再激活概率，并且具有弱初始Arc启动子激活的神经元在随后的训练期间被从集合中排除。我们的研究结果表明，在运动学习过程中，M2皮层的任务特异性弧依赖的细胞巩固过程。
Motor behaviors recruit task-specific neuronal ensembles in motor cortices, which are consolidated over subsequent learning. However, little is known about the molecules that can identify the participating neurons and predict the outcomes of the consolidation process. Using a mouse rotarod-learning task, we showed that lesion or inactivation of the secondary motor (M2) cortex disrupts learning of skilled movements. We tracked the endogenous promoter activity of the neuronal activity-regulated gene Arc in individual M2 neurons during rotarod learning by in vivo two-photon imaging of a knock-in reporter. We found that task training initially recruits Arc-promoter-activated neurons and then consolidates them into a specific ensemble exhibiting persistent reactivation of Arc-promoter. The intensity of a neuron’s initial Arc-promoter activation predicts its reactivation probability and neurons with weak initial Arc-promoter activation are dismissed from the ensemble during subsequent training. Our findings demonstrate a task-specific Arc-dependent cellular consolidation process in M2 cortex during motor learning.
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