A Portable Microfluidic System for Point-of-Care Detection of Multiple Protein Biomarkers.
A Portable Microfluidic System for Point-of-Care Detection of Multiple Protein Biomarkers.
复制标题
一种用于多种蛋白质生物标志物的即时检测的便携式微流体系统。
DOI：
10.3390/mi12040347
复制
发表时间：
2021-03-24
期刊：
影响因子：
3.4
通讯作者：
Xu Y
中科院分区：
文献类型：
--
作者：
Li N;Shen M;Xu Y
蛋白质生物标志物是多种疾病的标志物，在临床上常用于疾病诊断和预后预测。对蛋白质生物标志物的护理点(POC)检测的迫切需求促进了自动化和完全密封的免疫分析平台的发展。在本研究中，通过将用于多重免疫分析的蛋白质微阵列和用于试剂储存和液体操作的微流控小盒相结合，建立了便携式微流控系统，用于多个蛋白质生物标志物的POC检测。免疫分析的整个过程是自动进行的，包括抗体-抗原反应、洗涤和检测。该系统可在40min内同时检测甲胎蛋白、癌胚抗原和癌抗原125，检出限分别为0.303 ng/m L、1.870 ng/m L和18.617 U/L。采集了5份临床标本进行检验，结果与医院商用仪器测定的结果具有良好的相关性。免疫测定盒系统可以作为一个通用的平台，快速、灵敏地检测生物标志物；因此，它在POC诊断方面具有巨大的潜力。
Protein biomarkers are indicators of many diseases and are commonly used for disease diagnosis and prognosis prediction in the clinic. The urgent need for point-of-care (POC) detection of protein biomarkers has promoted the development of automated and fully sealed immunoassay platforms. In this study, a portable microfluidic system was established for the POC detection of multiple protein biomarkers by combining a protein microarray for a multiplex immunoassay and a microfluidic cassette for reagent storage and liquid manipulation. The entire procedure for the immunoassay was automatically conducted, which included the antibody–antigen reaction, washing and detection. Alpha-fetoprotein (AFP), carcinoembryonic antigen (CEA) and carcinoma antigen 125 (CA125) were simultaneously detected in this system within 40 min with limits of detection of 0.303 ng/mL, 1.870 ng/mL, and 18.617 U/mL, respectively. Five clinical samples were collected and tested, and the results show good correlations compared to those measured by the commercial instrument in the hospital. The immunoassay cassette system can function as a versatile platform for the rapid and sensitive multiplexed detection of biomarkers; therefore, it has great potential for POC diagnostics.
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DOI：
10.1016/j.gpb.2017.03.001
发表时间：
2017-04
期刊：
Genomics, proteomics & bioinformatics
影响因子：
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影响因子：
7.4
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影响因子：
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