Cellular performance comparison of biomimetic calcium phosphate coating and alkaline-treated titanium surface.
Cellular performance comparison of biomimetic calcium phosphate coating and alkaline-treated titanium surface.
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DOI：
10.1155/2013/832790
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发表时间：
2013
影响因子：
--
通讯作者：
Wei M
中科院分区：
文献类型：
--
作者：
Yu X;Wei M
仿生磷酸钙涂层对成骨细胞体外行为的影响尚不清楚。在这项研究中，我们研究了成骨细胞大鼠骨肉瘤17/2.8细胞(ROS17/2.8)在两组生物材料表面上的行为：碱性处理的钛表面(ATT)和仿生的磷酸钙涂层ATT(CAP)。对细胞在这些表面的附着、增殖、分化和形态进行了广泛的评估，以揭示基质表面对成骨细胞反应的影响。结果发现，培养在ATT表面的ROS17/2.8细胞的贴壁率和增殖率高于在帽面培养的ROS17/2.8细胞。我们的结果还表明，与ATT表面相比，本工作制备的磷酸钙涂层对成骨细胞的黏附具有抑制作用，并进一步影响成骨细胞的增殖和分化。培养两周后，ATT表面细胞的碱性磷酸酶活力也高于帽面细胞。免疫荧光染色和扫描电子显微镜结果显示，细胞在ATT表面的黏附和扩散速度快于在帽面。这些结果表明，基质的表面性质直接影响细胞在不同生物材料上的黏附，从而进一步影响细胞的增殖和分化。
The influence of biomimetic calcium phosphate coating on osteoblasts behavior in vitro is not well established yet. In this study, we investigated the behavior of osteoblastic rat osteosarcoma 17/2.8 cells (ROS17/2.8) on two groups of biomaterial surfaces: alkaline-treated titanium surface (ATT) and biomimetic calcium phosphate coated ATT (CaP). The cell attachment, proliferation, differentiation, and morphology on these surfaces were extensively evaluated to reveal the impact of substrate surface on osteoblastic cell responses. It was found that the ROS17/2.8 cells cultured on the ATT surface had higher attachment and proliferation rates compared to those on the CaP surface. Our results also showed that the calcium phosphate coatings generated in this work have an inhibiting effect on osteoblast adhesion and further influenced the proliferation and differentiation of osteoblast compared to the ATT surface in vitro. Cells on the ATT surface also exhibited a higher alkaline phosphatase activity than on the CaP surface after two weeks of culture. Immunofluorescence staining and scanning electron microscopy results showed that the cells adhered and spread faster on the ATT surface than on the CaP surface. These results collectively suggested that substrate surface properties directly influence cell adhesion on different biomaterials, which would result in further influence on the cell proliferation and differentiation.
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影响因子：
14
作者：
Lee, YJ;Ko, JS;Kim, HM
通讯作者：
Kim, HM
DOI：
10.1007/s10856-007-3347-4
发表时间：
2008-06-01
影响因子：
3.7
作者：
dos Santos, E. A.;Farina, M.;Anselme, K.
通讯作者：
Anselme, K.
通过表面纳米粗糙度调节细胞行为
影响因子：
--
作者：
Luo, Chao;Li, Long;Zhou, Shaobing
通讯作者：
Zhou, Shaobing
影响因子：
4.3
作者：
Gottlander, M;Johansson, CB;Albrektsson, T
通讯作者：
Albrektsson, T
影响因子：
4.1
作者：
Meleti, Z;Shapiro, IM;Adams, CS
通讯作者：
Adams, CS