Adverse effect of nano-silicon dioxide on lung function of rats with or without ovalbumin immunization.
Adverse effect of nano-silicon dioxide on lung function of rats with or without ovalbumin immunization.
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纳米二氧化硅对卵清蛋白免疫大鼠肺功能的不良影响。
DOI：
10.1371/journal.pone.0017236
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发表时间：
2011-02-17
期刊：
影响因子：
3.7
通讯作者：
Yang X
中科院分区：
文献类型：
--
作者：
Han B;Guo J;Abrahaley T;Qin L;Wang L;Zheng Y;Li B;Liu D;Yao H;Yang J;Li C;Xi Z;Yang X
纳米材料的巨大进步带来了广泛的重要应用，但其可能的纳米毒性和风险尚未得到充分理解。环境颗粒物（PM），尤其是超细颗粒物的暴露是导致肺功能损害和肺部疾病加重的重要原因。然而，纳米颗粒对过敏性哮喘的不良影响很少被研究，其机制仍不清楚。这项工作首次研究了过敏性哮喘与纳米二氧化硅（nano-SiO2）之间的关系。卵清蛋白（OVA）处理的和盐水处理的对照大鼠每天分别经气管内给予0.1 ml 0、40和80 μg/ml纳米SiO2溶液，持续30天。增加纳米SiO2暴露导致吸气和呼气阻力（Ri和Re）的不利变化，但对大鼠肺动态顺应性（Cldyn）的影响不明显。肺组织学观察显示，80 μg/ml纳米SiO2导入的生理盐水和OVA组的气道重塑明显，但后者更糟。此外，增加纳米SiO2暴露也会导致更严重的炎症。随着nano-SiO2暴露量的增加，肺组织匀浆中IL-4水平升高，IFN-γ水平降低，但变化不明显。此外，在相同的纳米SiO2暴露浓度下，与盐水处理的大鼠相比，OVA处理的大鼠表现出更高（显著）的IL-4和更低（不显著）的IFN-γ。嗜酸性粒细胞的百分比显示出非预期结果，其中暴露量越高，嗜酸性粒细胞百分比越低。本研究首次初步探讨了纳米SiO2对卵清蛋白诱导的大鼠哮喘模型的影响。结果提示，纳米SiO_2经气管内给药后，无论有无卵清蛋白免疫，均可引起气道高反应性（AHR）和气道重塑。这种情况可能是由于纳米SiO2通过增加组织IL-4的产生而加速了Th 1/Th 2细胞因子的失衡。
The great advances of nanomaterials have brought out broad important applications, but their possible nanotoxicity and risks have not been fully understood. It is confirmed that exposure of environmental particulate matter (PM), especially ultrafine PM, are responsible for many lung function impairment and exacerbation of pre-existing lung diseases. However, the adverse effect of nanoparticles on allergic asthma is seldom investigated and the mechanism remains undefined. For the first time, this work investigates the relationship between allergic asthma and nanosized silicon dioxide (nano-SiO2). Ovalbumin (OVA)-treated and saline-treated control rats were daily intratracheally administered 0.1 ml of 0, 40 and 80 µg/ml nano-SiO2 solutions, respectively for 30 days. Increased nano-SiO2 exposure results in adverse changes on inspiratory and expiratory resistance (Ri and Re), but shows insignificant effect on rat lung dynamic compliance (Cldyn). Lung histological observation reveals obvious airway remodeling in 80 µg/ml nano-SiO2-introduced saline and OVA groups, but the latter is worse. Additionally, increased nano-SiO2 exposure also leads to more severe inflammation. With increasing nano-SiO2 exposure, IL-4 in lung homogenate increases and IFN-γ shows a reverse but insignificant change. Moreover, at a same nano-SiO2 exposure concentration, OVA-treated rats exhibit higher (significant) IL-4 and lower (not significant) IFN-γ compared with the saline-treated rats. The percentages of eosinophil display an unexpected result, in which higher exposure results lower eosinophil percentages. This was a preliminary study which for the first time involved the effect of nano-SiO2 to OVA induced rat asthma model. The results suggested that intratracheal administration of nano-SiO2 could lead to the airway hyperresponsiveness (AHR) and the airway remolding with or without OVA immunization. This occurrence may be due to the Th1/Th2 cytokine imbalance accelerated by the nano-SiO2 through increasing the tissue IL-4 production.
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影响因子：
5.6
作者：
Park, Hee-ju;Lee, Chang-Min;Park, Yeong-Min
通讯作者：
Park, Yeong-Min
影响因子：
6.7
作者：
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影响因子：
10.4
作者：
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作者：
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作者：
Qiao, Y.;Li, B.;Yang, X.
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