Biogenesis of endoplasmic reticulum membranes. II. Synthesis of constitutive microsomal enzymes in developing rat hepatocyte.
Biogenesis of endoplasmic reticulum membranes. II. Synthesis of constitutive microsomal enzymes in developing rat hepatocyte.
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DOI：
10.1083/jcb.30.1.97
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发表时间：
1966-07
期刊：
影响因子：
--
通讯作者：
Palade GE
中科院分区：
文献类型：
--
作者：
Dallner G;Siekevitz P;Palade GE
在大鼠肝细胞内质网快速发育期间（出生前 3 天至出生后 8 天）研究了微粒体膜的组成酶。研究的活性（电子传递酶和磷酸酶）出现在不同的时间并以不同的速率增加。所测试的酶活性的增加被放线菌素D和嘌呤霉素抑制。 G-6-Pase 和 NADPH-细胞色素 c 还原酶活性首先出现在粗糙微粒体中，随后出现在光滑微粒体中，最终达到与成人肝脏中一样的均匀浓度。有证据表明酶在内质网的粗糙部分合成，然后转移到内质网的光滑部分。母体饮食中脂肪补充的变化导致微粒体磷脂的脂肪酸组成发生变化，但不影响乳儿的酶促模式。丙酮-H2O (10:1) 提取后，8 日龄和成年大鼠的微粒体失去了 95% 的 PLP 和 80% 的 NADH-细胞色素 c 还原酶活性。然而，通过添加由纯化的磷脂、心脏线粒体的脂质提取物、或8天或成年大鼠的肝微粒体制备的磷脂胶束，可以恢复一半的原始活性，从而证明非特异性磷脂对酶的激活。结果表明，在发育过程中，酶促模式不受内质网膜的脂肪酸或磷脂组成的影响。
The constitutive enzymes of microsomal membranes were investigated during a period of rapid ER development (from 3 days before to 8 days after birth) in rat hepatocytes. The activities studied (electron transport enzymes and phosphatases) appear at different times and increase at different rates. The increase in the enzyme activities tested was inhibited by Actinomycin D and puromycin. G-6-Pase and NADPH-cytochrome c reductase activities appeared first in the rough microsomes, and subsequently in smooth microsomes, eventually reaching a uniform concentration as in adult liver. The evidence suggests that the enzymes are synthesized in the rough part, then transferred to the smooth part, of the ER. Changes in the fat supplement of the maternal diet brought about changes in the fatty acid composition of microsomal phospholipids but did not influence the enzymic pattern of the suckling. Microsomes from 8-day-old and adult rats lose 95% of PLP and 80% of NADH-cytochrome c reductase activity after acetone-H2O (10:1) extraction. However, one-half the original activity could be regained by adding back phospholipid micelles prepared from purified phospholipid, or from lipid extracts of heart mitochondria, or of liver microsomes of 8-day or adult rats, thus demonstrating an activation of the enzyme by nonspecific phospholipid. The results suggest that during development the enzymic pattern is not influenced by the fatty acid or phospholipid composition of ER membranes.
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影响因子：
4.1
作者：
BIRAN, LA;RENSHAW, A;BARTLEY, W
通讯作者：
BARTLEY, W
DOI：
10.1016/0304-4165(64)90125-4
发表时间：
1964-01-01
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
EMMELOT, P;RUMKE, P;BENEDETTI, EL
通讯作者：
BENEDETTI, EL
影响因子：
56.9
作者：
Bernheim, F;von Felsovanyi, A
通讯作者：
von Felsovanyi, A
影响因子：
3.7
作者：
DAMELIO, V;MUTOLO, V;PIAZZA, E
通讯作者：
PIAZZA, E
影响因子：
2.9
作者：
DAS, ML;CRANE, FL
通讯作者：
CRANE, FL