Cyclic Nucleotide-Gated Ion Channel 6 Mediates Thermotolerance in Arabidopsis Seedlings by Regulating Hydrogen Peroxide Production via Cytosolic Calcium Ions.
Cyclic Nucleotide-Gated Ion Channel 6 Mediates Thermotolerance in Arabidopsis Seedlings by Regulating Hydrogen Peroxide Production via Cytosolic Calcium Ions.
复制标题
环状核苷酸门控离子通道 6 通过细胞溶质钙离子调节过氧化氢的产生来介导拟南芥幼苗的耐热性
DOI：
10.3389/fpls.2021.708672
复制
发表时间：
2021
影响因子：
5.6
通讯作者：
Zhao L
中科院分区：
文献类型：
--
作者：
Wang W;Zhang J;Ai L;Wu D;Li B;Zhang L;Zhao L
我们先前报道了环核苷酸门控离子通道6（CNGC 6）和过氧化氢（H2 O2）参与植物对热激（HS）的反应。为了证明它们与植物耐热性的关系，我们评估了HS对几组拟南芥（拟南芥）幼苗的影响：野生型，cngc 6突变体，及其互补线。在HS胁迫下，cngc 6突变体幼苗的H2 O2水平低于野生型（WT）幼苗，而互补株系的H2 O2水平明显升高。用钙离子（Ca 2+）处理拟南芥种子增加了HS处理下幼苗中的H2 O2水平，而用Ca 2+螯合剂（EGTA）处理则抑制了H2 O2水平，表明CNGC 6可能以依赖于胞质Ca 2+（[Ca 2 +]cyt）增加的方式刺激H2 O2的积累。这一点得到了验证，表型观察和耐热性测试与转基因植物过表达AtRbohB和AtRbohD（两个基因参与HS响应H2 O2生产），分别在cngc 6背景。实时荧光定量RT-PCR和Western blotting结果表明，CNGC 6通过H2 O2促进了HS因子（HSFs）的基因转录和HS蛋白（HSPs）的积累。这些结果表明，H2 O2在HS信号通路中作用于CNGC 6的下游，增加了我们对植物对高温反应的起始的理解。
We previously reported the involvement of cyclic nucleotide-gated ion channel 6 (CNGC6) and hydrogen peroxide (H2O2) in plant responses to heat shock (HS). To demonstrate their relationship with plant thermotolerance, we assessed the effect of HS on several groups of Arabidopsis (Arabidopsis thaliana) seedlings: wild-type, cngc6 mutant, and its complementation line. Under exposure to HS, the level of H2O2 was lower in the cngc6 mutant seedlings than in the wild-type (WT) seedlings but obviously increased in the complementation line. The treatment of Arabidopsis seeds with calcium ions (Ca2+) increased the H2O2 levels in the seedlings under HS treatment, whereas treatment with a Ca2+ chelator (EGTA) inhibited it, indicating that CNGC6 may stimulate the accumulation of H2O2 in a manner dependent on an increase in cytosolic Ca2+ ([Ca2+]cyt). This point was verified by phenotypic observations and thermotolerance testing with transgenic plants overexpressing AtRbohB and AtRbohD (two genes involved in HS-responsive H2O2 production), respectively, in a cngc6 background. Real-time reverse transcription-polymerase chain reactions and Western blotting suggested that CNGC6 enhanced the gene transcription of HS factors (HSFs) and the accumulation of HS proteins (HSPs) via H2O2. These upon results indicate that H2O2 acts downstream of CNGC6 in the HS signaling pathway, increasing our understanding of the initiation of plants responses to high temperatures.
登录
查看更多内容
影响因子：
4.3
作者：
Li, Ning;Sun, Lirong;Hao, Fu Shun
通讯作者：
Hao, Fu Shun
影响因子：
7.3
作者：
Liu, Hong-Tao;Li, Guo-Liang;Li, Bing
通讯作者：
Li, Bing
影响因子：
4.3
作者：
Macpherson, Neil;Takeda, Seiji;Davies, Julia M.
通讯作者：
Davies, Julia M.
影响因子：
7.3
作者：
Liu, HT;Sun, DY;Zhou, RG
通讯作者：
Zhou, RG
影响因子：
7.3
作者：
Gupta, D. K.;Pena, L. B.;Sandalio, L. M.
通讯作者：
Sandalio, L. M.