Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin a in model cell membranes.
Mass spectral imaging of glycophospholipids, cholesterol, and glycophorin a in model cell membranes.
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DOI：
10.1021/la802582f
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发表时间：
2008-10-21
期刊：
影响因子：
3.9
通讯作者：
Vickerman, John C.
中科院分区：
文献类型：
--
作者：
Baker, Matthew J.;Zheng, Leiliang;Winograd, Nicholas;Lockyer, Nicholas P.;Vickerman, John C.
飞行时间二次离子质谱（ToF-SIMS）和LB技术已被用于创建和分析可重复的膜模拟的细胞膜的内部和外部小叶，以研究脂质-蛋白质和脂质-脂质相互作用。利用由磷脂、胆固醇和完整膜蛋白组成的膜。结果表明，外膜小叶模拟物（DPPC/胆固醇/血型糖蛋白A LB膜）由单一均相组成，而内膜小叶模拟物（DPPE/胆固醇/血型糖蛋白A LB膜）以两个分离相的形式显示异质性。DPPE/胆固醇相和血型糖蛋白A相。这表明基于细胞膜小叶的不同化学组成的膜结构域形成的差异。通过建立矩阵效应对测量的影响，并利用PCA增强图像的对比度，提高了测量的可靠性。
Time of flight secondary ion mass spectrometry (ToF-SIMS) and the Langmuir—Blodgett (LB) technique have been used to create and analyze reproducible membrane mimics of the inner and outer leaflets of a cellular membrane to investigate lipid—protein and lipid—lipid interactions. Films composed of phospholipids, cholesterol and an integral membrane protein were utilized. The results show the outer membrane leaflet mimic (DPPC/cholesterol/glycophorin A LB film) consisting of a single homogeneous phase whereas the inner membrane leaflet mimic (DPPE/cholesterol/glycophorin A LB film) displays heterogeneity in the form of two separate phases. A DPPE/cholesterol phase and a glycophorin A phase. This points to differences in membrane domain formation based upon the different chemical composition of the leaflets of a cell membrane. The reliability of the measurements was enhanced by establishing the influence of the matrix effect upon the measurement and by utlilizing PCA to enhance the contrast of the images.
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