Organ specificity in the plant circadian system is explained by different light inputs to the shoot and root clocks.
Organ specificity in the plant circadian system is explained by different light inputs to the shoot and root clocks.
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DOI：
10.1111/nph.14024
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发表时间：
2016-10
期刊：
影响因子：
--
通讯作者：
Nimmo HG
中科院分区：
文献类型：
--
作者：
Bordage S;Sullivan S;Laird J;Millar AJ;Nimmo HG
昼夜节律时钟允许生物过程的时间划分。在拟南芥中，昼夜节律表现出器官特异性，但潜在的分子原因尚未确定。我们研究了在恒定条件和光：暗循环下成熟芽和根的时钟之间的相似性和差异的机制。我们开发了一种成像系统来监测芽和光或暗生长的根中的时钟基因表达，修改了拟南芥时钟的最新数学模型，并用它来模拟我们的新数据。我们表明，芽和根的生物钟具有不同的节律特性（周期和幅度），并且对光质量的响应不同。直接暴露在低强度光下，即使与芽的照明反相，也会影响根时钟。在有光的条件下，时钟之间的差异比在持续黑暗的条件下更为明显，并且在存在蔗糖的情况下持续存在。我们通过修改与光输入相关的时钟模型参数成功地模拟了数据。我们的结论是，芽和根时钟之间的差异和相似之处很大程度上可以通过器官特异性光输入来解释。这为器官特异性时钟的发展领域提供了机制上的见解。
Circadian clocks allow the temporal compartmentalization of biological processes. In Arabidopsis, circadian rhythms display organ specificity but the underlying molecular causes have not been identified. We investigated the mechanisms responsible for the similarities and differences between the clocks of mature shoots and roots in constant conditions and in light : dark cycles. We developed an imaging system to monitor clock gene expression in shoots and light‐ or dark‐grown roots, modified a recent mathematical model of the Arabidopsis clock and used this to simulate our new data. We showed that the shoot and root circadian clocks have different rhythmic properties (period and amplitude) and respond differently to light quality. The root clock was entrained by direct exposure to low‐intensity light, even in antiphase to the illumination of shoots. Differences between the clocks were more pronounced in conditions where light was present than in constant darkness, and persisted in the presence of sucrose. We simulated the data successfully by modifying those parameters of a clock model that are related to light inputs. We conclude that differences and similarities between the shoot and root clocks can largely be explained by organ‐specific light inputs. This provides mechanistic insight into the developing field of organ‐specific clocks.
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