Adaptive response to wine selective pressures shapes the genome of a Saccharomyces interspecies hybrid.
Adaptive response to wine selective pressures shapes the genome of a Saccharomyces interspecies hybrid.
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DOI：
10.1099/mgen.0.000628
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发表时间：
2021-08
影响因子：
3.9
通讯作者：
Querol A
中科院分区：
文献类型：
--
作者：
Lairón-Peris M;Castiglioni GL;Routledge SJ;Alonso-Del-Real J;Linney JA;Pitt AR;Melcr J;Goddard AD;Barrio E;Querol A
在工业过程中，酵母暴露在恶劣的条件下，最终导致菌株的适应。在实验室中，有可能使用实验进化将进化生物学反应与特定酵母菌株的工业改进的这些适应压力联系起来。在这项工作中，我们旨在研究葡萄酒工业酵母在香槟生产过程中停止发酵和二次发酵过程中高浓度乙醇的应激条件下的适应性。我们使用了一种商业化的酿酒酵母× S. uvarum杂交品种，并评估了它在含有高乙醇的改性合成must （M-SM）中的适应性，其中还含有代谢亚硫酸盐，这是一种在葡萄酒发酵过程中转化为亚硫酸盐时使用的防腐剂。在这些选定的应激环境条件下，对适应菌株（H14A7-etoh）对亚硫酸盐和乙醇的耐受性进行了研究，结果表明，与原菌株H14A7相比，适应杂交种对亚硫酸盐和乙醇的耐受性增强，而对乙醇的耐受性略有提高。然而，在适应性杂交中发现了一种权衡，因为与H14A7相比，它发酵葡萄糖和果糖的能力较低。杂种基因组几乎总是不稳定的，在H14A7-etoh基因组上检测到不同的适应信号。在适应菌株中存在的每个亚基因组都有不同的适应。酿酒葡萄球菌的第3号染色体和已复制的uvarum的第7 ~ 16号染色体存在染色体非整倍体。此外，猪链球菌I号染色体不存在于H14A7-etoh中，酿酒链球菌I号染色体出现了杂合性缺失（LOH）事件。rna测序分析显示H14A7-etoh和H14A7基因表达差异，这很容易与在H14A7-etoh基因组上发现的适应信号相关联。最后，我们报告了膜脂质组成的改变，与保守的耐受性机制一致。
During industrial processes, yeasts are exposed to harsh conditions, which eventually lead to adaptation of the strains. In the laboratory, it is possible to use experimental evolution to link the evolutionary biology response to these adaptation pressures for the industrial improvement of a specific yeast strain. In this work, we aimed to study the adaptation of a wine industrial yeast in stress conditions of the high ethanol concentrations present in stopped fermentations and secondary fermentations in the processes of champagne production. We used a commercial Saccharomyces cerevisiae × S. uvarum hybrid and assessed its adaptation in a modified synthetic must (M-SM) containing high ethanol, which also contained metabisulfite, a preservative that is used during wine fermentation as it converts to sulfite. After the adaptation process under these selected stressful environmental conditions, the tolerance of the adapted strain (H14A7-etoh) to sulfite and ethanol was investigated, revealing that the adapted hybrid is more resistant to sulfite compared to the original H14A7 strain, whereas ethanol tolerance improvement was slight. However, a trade-off in the adapted hybrid was found, as it had a lower capacity to ferment glucose and fructose in comparison with H14A7. Hybrid genomes are almost always unstable, and different signals of adaptation on H14A7-etoh genome were detected. Each subgenome present in the adapted strain had adapted differently. Chromosome aneuploidies were present in S. cerevisiae chromosome III and in S. uvarum chromosome VII–XVI, which had been duplicated. Moreover, S. uvarum chromosome I was not present in H14A7-etoh and a loss of heterozygosity (LOH) event arose on S. cerevisiae chromosome I. RNA-sequencing analysis showed differential gene expression between H14A7-etoh and H14A7, which can be easily correlated with the signals of adaptation that were found on the H14A7-etoh genome. Finally, we report alterations in the lipid composition of the membrane, consistent with conserved tolerance mechanisms.
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DOI：
10.1093/bioinformatics/btu638
发表时间：
2015-01-15
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Anders S;Pyl PT;Huber W
通讯作者：
Huber W
影响因子：
4
作者：
Chi, Z;Arneborg, N
通讯作者：
Arneborg, N
影响因子：
4.8
作者：
Coleman, ST;Fang, TK;Moye-Rowley, WS
通讯作者：
Moye-Rowley, WS
影响因子：
4.8
作者：
Dawaliby, Rosie;Trubbia, Cataldo;Govaerts, Cedric
通讯作者：
Govaerts, Cedric
影响因子：
2.5
作者：
CASALONE, E;COLELLA, CM;POLSINELLI, M
通讯作者：
POLSINELLI, M