Thrombospondin-1 expression in urothelial carcinoma: prognostic significance and association with p53 alterations, tumour angiogenesis and extracellular matrix components.
Thrombospondin-1 expression in urothelial carcinoma: prognostic significance and association with p53 alterations, tumour angiogenesis and extracellular matrix components.
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DOI：
10.1186/1471-2407-6-140
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发表时间：
2006-05-29
期刊：
影响因子：
3.8
通讯作者：
Stavropoulos NE
中科院分区：
文献类型：
--
作者：
Ioachim E;Michael MC;Salmas M;Damala K;Tsanou E;Michael MM;Malamou-Mitsi V;Stavropoulos NE
血小板反应蛋白-1（TSP-1）是细胞外基质成分糖蛋白，已知其是血管生成的有效抑制剂，并且在癌症侵袭中可能是重要的。我们研究了TSP-1表达与传统临床病理参数的相关性，以阐明其在膀胱癌中的预后意义。此外，TSP-1表达与微血管计数，VEGF表达，p53表达以及与细胞外基质成分的表达的可能相关性进行了研究，以探讨其在血管化和肿瘤间质重塑中的意义。在148例福尔马林固定石蜡包埋的尿路上皮细胞癌组织样本中，研究了TSP-1在肿瘤细胞和肿瘤间质中的免疫组化表达。TSP-1在血管周围组织中检测到，在上皮-基质交界处，在基质和肿瘤细胞中的大多数情况下。在肿瘤细胞中，43%的病例中观察到TSP-1低表达，7%的病例中中高表达，而50%的病例显示TSP表达缺失。高TSP-1免疫反应性在良好和中等分化的肿瘤相比，分化不良。与pTa和pT 2 -4肿瘤相比，PT 1肿瘤显示TSP-1表达降低。肿瘤细胞TSP-1表达增加与微血管密度增加有关。在肿瘤间质中，37%的病例显示少量TSP-1表达，7.5%的病例显示中度和高度表达，而55%的病例显示TSP-1间质免疫反应性缺失。间质TSP-1表达与肿瘤分期和肿瘤大小呈负相关。这种表达也与微血管密度、VEGF表达和细胞外基质成分腱生蛋白和纤维连接蛋白呈正相关。使用单变量和多变量分析，我们没有发现任何显着的相关性，TSP-1表达在浅表肿瘤的肿瘤细胞和肿瘤间质的复发和疾病进展的风险的风险。我们的数据表明，肿瘤和间质TSP-1表达可能发挥作用，在肿瘤的侵袭性和血管生成。此外，间质TSP-1表达与细胞外基质成分纤连蛋白和腱生蛋白的相关性表明其可能与肿瘤间质重塑有关。
Thrombospondin-1 (TSP-1) is an extracellular matrix component glycoprotein, which is known to be a potent inhibitor of angiogenesis and may be important in cancer invasiveness. We examined the TSP-1 expression in correlation with conventional clinicopathological parameters to clarify its prognostic significance in bladder cancer. In addition, the possible correlation of TSP-1 expression with microvessel count, VEGF expression, p53 expression as well as with the expression of the extracellular matrix components was studied to explore its implication in vascularization and tumour stroma remodeling. The immunohistochemical expression of TSP-1 in tumour cells and in the tumour stroma was studied in 148 formalin-fixed paraffin-embedded urothelial cell carcinoma tissue samples. TSP-1 was detected in perivascular tissue, at the epithelial-stromal junction, in the stroma and in tumour cells in the majority of the cases. In tumour cells, low TSP-1 expression was observed in 43% of the cases, moderate and high in 7%, while 50% showed absence of TSP expression. A higher TSP-1 immunoreactivity in well and moderately differentiated tumours compared to poorly differentiated was noted. PT1 tumours showed decreased TSP-1 expression in comparison to pTa and pT2–4 tumours. Increased tumour cell TSP-1 expression was related to increased microvessel density. In the tumour stroma, 37% of the cases showed small amount of TSP-1 expression, 7.5% moderate and high, while 55% of the cases showed absence of TSP-1 stromal immunoreactivity. Stromal TSP-1 expression was inversely correlated with tumour stage and tumour size. This expression was also positively correlated with microvessel density, VEGF expression and extracellular matrix components tenascin and fibronectin. Using univariate and multivariate analysis we didn't find any significant correlation of TSP-1 expression in superficial tumours in both tumour cells and tumour stroma in terns of the risk of recurrence and disease progression Our data suggest that both tumour and stromal TSP-1 expression may play a role in tumour aggressiveness and angiogenesis. In addition, the correlation of stromal TSP-1 expression with extracellular matrix components fibronectin and tenascin indicate its possible implication in tumour stroma remodeling.
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DOI：
10.1016/0922-3371(90)90057-4
发表时间：
1990-12-01
期刊：
CELL DIFFERENTIATION AND DEVELOPMENT
影响因子：
--
作者：
GOULD, VE;KOUKOULIS, GK;VIRTANEN, I
通讯作者：
VIRTANEN, I
影响因子：
8.4
作者：
Ioachim, E;Charchanti, A;Pavlidis, N
通讯作者：
Pavlidis, N
影响因子：
2.5
作者：
CORLESS, CL;MENDOZA, A;LAWLER, J
通讯作者：
LAWLER, J
影响因子：
3.5
作者：
Gasparini, G;Toi, M;Gion, M
通讯作者：
Gion, M
影响因子：
4.5
作者：
Ioachim, E;Michael, M;Malamou-Mitsi, V
通讯作者：
Malamou-Mitsi, V