Differences in gene expression between the otic capsule and other bones.
Differences in gene expression between the otic capsule and other bones.
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DOI：
10.1016/j.heares.2010.02.006
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发表时间：
2010-06-14
期刊：
影响因子：
2.8
通讯作者：
McKenna, Michael J.
中科院分区：
文献类型：
--
作者：
Stankovic, Konstantina M.;Adachi, Osamu;Tsuji, Kunikazu;Kristiansen, Arthur G.;Adams, Joe C.;Rosen, Vicki;McKenna, Michael J.
我们的长期目标是了解耳硬化症的分子病理学，并开发更好的治疗方法。为了实现这一目标，目前的研究重点是表征负责耳囊独特生物学特征的分子因素：其重塑率最低，以及骨折时缺乏愈合能力。我们比较了62个基因的表达水平，参与骨代谢之间的成年小鼠耳囊和胫骨和顶骨，后者骨形成的软骨内和膜内骨化，分别。采用实时定量RT-PCR方法检测基因表达水平，并利用生物信息学工具进行分析。原位杂交验证关键基因的表达模式。耳囊的分子谱与胫骨和顶骨的分子谱明显不同。被发现最具耳囊特征的基因是：骨保护素（opg）、骨形态发生蛋白受体1b（bmpr 1b）和骨形态发生蛋白3（bmp 3）。opg和bmpr 1b的表达水平很高，而bmp 3在耳囊内的表达水平很低。我们的结论是，opg和bmpr 1b可能在抑制耳囊重塑中发挥重要作用。
Our long term goal is to understand the molecular pathology of otosclerosis and to develop better forms of therapy. Toward this goal, the current study focused on characterizing the molecular factors responsible for the unique biological features of the otic capsule: its minimal rate of remodeling, and lack of healing capacity when fractured. We compared expression levels of 62 genes involved in bone metabolism between the adult murine otic capsule and the tibia and parietal bones; the latter exemplify bones formed by endochondral and intramembranous ossification, respectively. Gene expression levels were measured using real-time quantitative RT-PCR and analyzed using tools of bioinformatics. Expression patterns of key genes were verified with in situ hybridization. The molecular profile of the otic capsule was distinctly different from that of the tibia and parietal bone. Genes found to be most characteristic of the otic capsule were: osteoprotegerin (opg), bone morphogenetic protein receptor 1b (bmpr1b) and bone morphogenetic protein 3 (bmp3). Expression levels were high for opg and bmpr1b, and minimal for bmp3 within the otic capsule. We concluded that opg and bmpr1b likely play important roles in inhibition of remodeling within the otic capsule.
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