Brief neonatal maternal separation alters extinction of conditioned fear and corticolimbic glucocorticoid and NMDA receptor expression in adult rats.
Brief neonatal maternal separation alters extinction of conditioned fear and corticolimbic glucocorticoid and NMDA receptor expression in adult rats.
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短暂的新生儿产妇分离改变了成年大鼠中条件恐惧和皮质糖皮质激素和NMDA受体表达的灭绝。
DOI：
10.1002/dneu.20691
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发表时间：
2009-02-01
影响因子：
3
通讯作者：
Wellman CL
中科院分区：
文献类型：
--
作者：
Wilber AA;Southwood CJ;Wellman CL
新生儿母亲分离改变成人HPA轴对压力的反应，成人情绪和前脑区域如海马的糖皮质激素受体（GR）浓度。为了研究新生儿母亲分离对情绪调节及其神经基质的影响，我们评估了经历新生儿母亲分离的成年大鼠条件性恐惧的获得和消退。包括基底外侧杏仁核和内侧前额叶皮质在内的皮质边缘结构对于条件性恐惧的获得和消退至关重要，并且这种学习是N-甲基D-天冬氨酸（NMDA）受体依赖的。因此，我们使用免疫组织化学方法来评估基底外侧杏仁核和内侧前额叶皮质中GR和NMDA受体NR 1亚单位的表达。在出生后第2-14天，幼崽进行控制饲养或母亲分离，每天15分钟。然后在雄性大鼠中评估成年期的恐惧条件反射和消退。大鼠接受了五次单独的音调习惯化试验，然后是七次音调/脚震配对。1小时后，大鼠接受单独的音调消退试验，以标准，和15回忆消退试验的第二天。对脑进行GR和NR 1的免疫组织化学标记，并对染色进行定量。短暂的母亲分离并没有改变收购或最初的灭绝，但受损的灭绝回忆。短暂的母亲分离并没有改变GR或NR 1在基底外侧杏仁核的表达。然而，短暂的母亲分离增加GR和减少NR 1的表达，特别是在内侧前额叶皮层的边缘下区，与工作涉及这方面的灭绝回忆。因此，短暂的母亲分离损害了消退回忆，并改变了成年人神经基质中的GR和NR 1表达。
Neonatal maternal separation alters adult HPA axis responsiveness to stress, adult emotionality, and glucocorticoid receptor (GR) concentrations in forebrain regions such as hippocampus. To investigate effects of neonatal maternal separation on emotion regulation and its neural substrates, we assessed acquisition and extinction of conditioned fear in adult rats that underwent neonatal maternal separation. Corticolimbic structures including basolateral amygdala and medial prefrontal cortex are critical for acquisition and extinction of conditioned fear, and such learning is N-methylD-aspartic acid (NMDA) receptor-dependent. Thus, we used immunohistochemistry to assess expression of the GR and the NR1 subunit of the NMDA receptor in basolateral amygdala and medial prefrontal cortex. On postnatal days 2–14, pups underwent control rearing or maternal separation for 15 min per day. Fear conditioning and extinction in adulthood were then assessed in male rats. Rats received five tone-alone habituation trials, then seven tone/footshock pairings. After 1 h, rats received tone-alone extinction trials to criterion, and 15 recall of extinction trials the next day. Brains were processed for immunohistochemical labeling of GR and NR1, and staining was quantified. Brief maternal separation did not alter acquisition or initial extinction, but impaired extinction recall. Brief maternal separation did not alter GR or NR1 expression in basolateral amygdala. However, brief maternal separation increased GR and decreased NR1 expression specifically in the infralimbic region of medial prefrontal cortex, consistent with work implicating this area in extinction recall. Thus, brief maternal separation impaired extinction recall and altered GR and NR1 expression in its neural substrate in adults.
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影响因子：
3.7
作者：
Aisa, Barbara;Tordera, Rosa;Ramirez, Maria J.
通讯作者：
Ramirez, Maria J.
影响因子：
2
作者：
Calandreau, L;Desmedt, A;Jaffard, R
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Jaffard, R
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2
作者：
Collins, DR;Paré, D
通讯作者：
Paré, D
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作者：
Caldji, C;Francis, D;Meaney, MJ
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Meaney, MJ
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作者：
Gibb, R;Kolb, B
通讯作者：
Kolb, B