Regulation of plant immunity through modulation of phytoalexin synthesis.
Regulation of plant immunity through modulation of phytoalexin synthesis.
复制标题
DOI：
10.3390/molecules19067480
复制
发表时间：
2014-06-06
期刊：
影响因子：
--
通讯作者：
Lozovaya VV
中科院分区：
文献类型：
--
作者：
Zernova OV;Lygin AV;Pawlowski ML;Hill CB;Hartman GL;Widholm JM;Lozovaya VV
大豆毛状根转化与白藜芦醇合酶和白藜芦醇氧甲基转移酶基因驱动的组成型拟南芥肌动蛋白和CsVMV启动子进行了表征。转化的毛状根积累了芪类化合物白藜芦醇和相关化合物紫檀芪的糖苷缀合物，这些化合物通常不由大豆植物合成。大豆毛状根中非天然芪类植物防御素合成的表达增加了它们对大豆病原体立枯丝核菌的抗性。与未转化的毛状根相比，AhRS3基因的表达导致根坏死减少20%至50%。两个基因的表达，AhRS3和ROMT，所需的pterostilbene在大豆中的合成，导致显着较低的根坏死（范围从0%至7%），在转基因根比未转化的毛状根，有约84%的坏死。大豆异戊烯基转移酶（二甲基烯丙基转移酶）G4DT基因在大豆毛状根中的过表达增加。
Soybean hairy roots transformed with the resveratrol synthase and resveratrol oxymethyl transferase genes driven by constitutive Arabidopsis actin and CsVMV promoters were characterized. Transformed hairy roots accumulated glycoside conjugates of the stilbenic compound resveratrol and the related compound pterostilbene, which are normally not synthesized by soybean plants. Expression of the non-native stilbenic phytoalexin synthesis in soybean hairy roots increased their resistance to the soybean pathogen Rhizoctonia solani. The expression of the AhRS3 gene resulted in 20% to 50% decreased root necrosis compared to that of untransformed hairy roots. The expression of two genes, the AhRS3 and ROMT, required for pterostilbene synthesis in soybean, resulted in significantly lower root necrosis (ranging from 0% to 7%) in transgenic roots than in untransformed hairy roots that had about 84% necrosis. Overexpression of the soybean prenyltransferase (dimethylallyltransferase) G4DT gene in soybean hairy roots increased.
登录
查看更多内容
影响因子：
5.6
作者：
Jeandet P;Clément C;Courot E;Cordelier S
通讯作者：
Cordelier S
影响因子：
6
作者：
Jeandet, Philippe;Delaunois, Bertrand;Courot, Eric
通讯作者：
Courot, Eric
影响因子：
4.3
作者：
Lozovaya, Vera V.;Lygin, Anatoliy V.;Widholm, Jack M.
通讯作者：
Widholm, Jack M.
影响因子：
5.4
作者：
Leckband, G;Lörz, H
通讯作者：
Lörz, H
影响因子：
2.3
作者：
Lozovaya, VV;Lygin, AV;Widhohn, JM
通讯作者：
Widhohn, JM