Curcumin Supplementation Ameliorates Bile Cholesterol Supersaturation in Hamsters by Modulating Gut Microbiota and Cholesterol Absorption.
Curcumin Supplementation Ameliorates Bile Cholesterol Supersaturation in Hamsters by Modulating Gut Microbiota and Cholesterol Absorption.
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姜黄素是一种多酚，已被证明具有益生元和降低胆固醇的特性。本研究旨在探讨姜黄素对胆汁胆固醇过饱和的影响，以及肠道菌群和胆固醇吸收的潜在机制。雄性仓鼠（n = 8）分别饲喂添加或不添加姜黄素的高脂肪饲粮（HFD） 12周。结果表明，姜黄素通过改变总胆汁酸（BAs）、磷脂和胆固醇的浓度，显著降低了hld喂养仓鼠的血清胆固醇水平（从5.10降至4.10 mmol/L）和肝脏胆固醇水平（从64.60降至47.72 nmol/mg protein），使胆胆固醇饱和指数（CSI）从1.64降至1.08 （p < 0.05）。通过16S rRNA测序对肠道菌群进行分析发现，姜黄素调节肠道菌群，主要是增加与BA代谢和短链脂肪酸产生相关的菌群，从而上调肝脏胆固醇7- α羟化酶的表达，增加胆汁酸的合成（p < 0.05）。此外，姜黄素显著下调仓鼠肠道尼曼-匹克c1样蛋白1（NPC1L1）的表达，降低Caco-2细胞对胆固醇的吸收（p < 0.05）。我们的研究结果表明，膳食姜黄素有可能通过调节肠道微生物群和抑制肠道胆固醇吸收来预防胆汁胆固醇过饱和。
Curcumin is a polyphenol that has been shown to have prebiotic and cholesterol-lowering properties. This study aimed to investigate the impact of curcumin on bile cholesterol supersaturation and the potential mechanistic role of intestinal microbiota and cholesterol absorption. Male hamsters (n = 8) were fed a high-fat diet (HFD) supplemented with or without curcumin for 12 weeks. Results showed that curcumin significantly decreased cholesterol levels in the serum (from 5.10 to 4.10 mmol/L) and liver (from 64.60 to 47.72 nmol/mg protein) in HFD-fed hamsters and reduced the bile cholesterol saturation index (CSI) from 1.64 to 1.08 due to the beneficial modifications in the concentration of total bile acids (BAs), phospholipids and cholesterol (p < 0.05). Gut microbiota analysis via 16S rRNA sequencing revealed that curcumin modulated gut microbiota, predominantly increasing microbiota associated with BA metabolism and short-chain fatty acid production, which subsequently up-regulated the expression of hepatic cholesterol 7-alpha hydroxylase and increased the synthesis of bile acids (p < 0.05). Furthermore, curcumin significantly down-regulated the expression of intestinal Niemann–Pick C1-like protein 1(NPC1L1) in hamsters and reduced cholesterol absorption in Caco-2 cells (p < 0.05). Our results demonstrate that dietary curcumin has the potential to prevent bile cholesterol supersaturation through modulating the gut microbiota and inhibiting intestinal cholesterol absorption.
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