Cell mechanical properties of human breast carcinoma cells depend on temperature.
Cell mechanical properties of human breast carcinoma cells depend on temperature.
复制标题
DOI：
10.1038/s41598-021-90173-y
复制
发表时间：
2021-05-24
影响因子：
4.6
通讯作者：
Mierke CT
中科院分区：
文献类型：
--
作者：
Aermes C;Hayn A;Fischer T;Mierke CT
细胞力学的知识需要了解细胞过程和功能，如细胞的运动，以及癌症治疗中组织工程的发展。细胞机械性能取决于多种因素，如细胞环境，也可能依赖于外部因素，如环境温度。温度对细胞力学的影响还不清楚。为了探索温度对细胞力学的影响，我们使用磁镊对4.5 μm的超顺磁珠施加1 nN的力。用纤连蛋白包被珠粒并将其偶联至人上皮乳腺癌细胞，特别是MCF-7和MDA-MB-231细胞。在25 ° C至45 °C的温度范围内测量电池。两种细胞类型的蠕变响应遵循弱幂律。在所有温度下，与MCF-7细胞相比，MDA-MB-231细胞明显更软，而它们的流动性增加。然而，随着温度的升高，细胞变得明显更软，更流畅。由于机械性质表现在细胞的细胞骨架结构中，并且顺磁珠通过连接到细胞骨架结构（如肌动蛋白和肌球蛋白丝以及微管）的细胞表面受体偶联，因此用影响肌动蛋白丝聚合的药理学药物（如Latrunculin A）、影响肌球蛋白丝聚合的药理学药物（如Blebbistatin）和影响微管聚合的药理学药物（如Demecolcine）探测细胞。在特定温度范围内的磁镊测量期间。无论药物干预，蠕变反应的细胞在所有温度下遵循弱幂律。肌动蛋白聚合的抑制导致两种细胞类型的柔软性增加，并且仅在MDA-MB-231细胞中流动性降低。Blebbistatin在低温下对MDA-MB-231细胞的顺应性有影响，对MCF-7细胞的顺应性影响较小。微管抑制影响MCF-7细胞的流动性，但对MCF-7和MDA-MB-231细胞的顺应性没有显著影响。总之，随着温度的升高，细胞变得明显更软，研究的药物和细胞系之间存在特定差异。
The knowledge of cell mechanics is required to understand cellular processes and functions, such as the movement of cells, and the development of tissue engineering in cancer therapy. Cell mechanical properties depend on a variety of factors, such as cellular environments, and may also rely on external factors, such as the ambient temperature. The impact of temperature on cell mechanics is not clearly understood. To explore the effect of temperature on cell mechanics, we employed magnetic tweezers to apply a force of 1 nN to 4.5 µm superparamagnetic beads. The beads were coated with fibronectin and coupled to human epithelial breast cancer cells, in particular MCF-7 and MDA-MB-231 cells. Cells were measured in a temperature range between 25 and 45 °C. The creep response of both cell types followed a weak power law. At all temperatures, the MDA-MB-231 cells were pronouncedly softer compared to the MCF-7 cells, whereas their fluidity was increased. However, with increasing temperature, the cells became significantly softer and more fluid. Since mechanical properties are manifested in the cell’s cytoskeletal structure and the paramagnetic beads are coupled through cell surface receptors linked to cytoskeletal structures, such as actin and myosin filaments as well as microtubules, the cells were probed with pharmacological drugs impacting the actin filament polymerization, such as Latrunculin A, the myosin filaments, such as Blebbistatin, and the microtubules, such as Demecolcine, during the magnetic tweezer measurements in the specific temperature range. Irrespective of pharmacological interventions, the creep response of cells followed a weak power law at all temperatures. Inhibition of the actin polymerization resulted in increased softness in both cell types and decreased fluidity exclusively in MDA-MB-231 cells. Blebbistatin had an effect on the compliance of MDA-MB-231 cells at lower temperatures, which was minor on the compliance MCF-7 cells. Microtubule inhibition affected the fluidity of MCF-7 cells but did not have a significant effect on the compliance of MCF-7 and MDA-MB-231 cells. In summary, with increasing temperature, the cells became significant softer with specific differences between the investigated drugs and cell lines.
登录
查看更多内容
影响因子：
5.1
作者：
Israel Farfan-Cabrera, Leonardo;Benito Pascual-Francisco, Juan;Susarrey-Huerta, Orlando
通讯作者：
Susarrey-Huerta, Orlando
影响因子：
4.4
作者：
Chan CJ;Whyte G;Boyde L;Salbreux G;Guck J
通讯作者：
Guck J
影响因子：
4.6
作者：
Diermeier S;Iberl J;Vetter K;Haug M;Pollmann C;Reischl B;Buttgereit A;Schürmann S;Spörrer M;Goldmann WH;Fabry B;Elhamine F;Stehle R;Pfitzer G;Winter L;Clemen CS;Herrmann H;Schröder R;Friedrich O
通讯作者：
Friedrich O
影响因子：
4.6
作者：
Jaferzadeh, Keyvan;Sim, MinWoo;Moon, Inkyu
通讯作者：
Moon, Inkyu
影响因子：
4.6
作者：
Fernandez-de-Cossio-Diaz J;Vazquez A
通讯作者：
Vazquez A