Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults.
Variability in response to vitamin D supplementation according to vitamin D metabolism related gene polymorphisms in healthy adults.
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DOI：
10.1038/s41430-022-01218-y
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发表时间：
2023-02
影响因子：
4.7
通讯作者：
中科院分区：
文献类型：
--
作者：
本研究的目的是确定维生素 D (vitD) 代谢途径的一些关键基因因子的多态性对补充剂功效的影响。萨卢尔大学医院职业医学服务中心总共招募了 245 名患有 vitD 缺乏 [25(OH)D ≤ 30ng/ml] 的健康参与者。在给予知情同意后，所有参与者都被要求填写一份通用调查问卷并遵循详细的个性化补充方案。通过PCR-RFLP对属于DBP、CYP2R1、CYP27B14、CYP24A1和VDR基因的15个单核苷酸多态性(SNP)进行遗传学研究。采用SPSS23.0进行统计研究。在所研究的 SNP 中，无反应与 rs4588 (OR* = 11.51; p < 0.001)、rs10766197 (OR* = 6.92; p = 0.008) 和 rs12794714 的变异等位基因显着相关（OR* = 5.09；p = 0.004）。这三个 SNP 在响应变异中贡献了 18.8%，其中 rs4588 影响最大 (10.3%)。基线 25(OH)D 和补充后 25(OH)D 浓度之间存在显着的线性负相关 (r = −0.437；p < 0.001)，并且 25(OH)D 浓度的增加与 GRS 之间存在线性负相关（GRS：遗传风险评分 = 风险等位基因之和） (r = −0.149；p = 0.033)。 DBP-rs4588、CYP2R1-rs10766197 和 rs12794714 变异体与突尼斯成年人血清 25(OH)D 浓度的变化以及对 vitD 补充剂的反应功效相关。考虑到这些变化有助于更好地调整维生素 D 摄入量，以确保对补充剂有更高的反应。
The aim of this study was to determine the influence of polymorphisms in some key gene actors of the vitamin D (vitD) metabolic pathway on supplementation efficacy. In total, 245 healthy participants were recruited from occupational medicine service in Sahloul University Hospital with vitD deficiency [25(OH)D ≤ 30 ng/ml]. After giving an informed consent, all participants were asked to complete a generalized questionnaire and to follow a detailed personalized supplementation protocol. Genetic study was performed by PCR-RFLP for 15 single nucleotide polymorphisms (SNPs) belonging to DBP, CYP2R1, CYP27B14, CYP24A1 and VDR genes. Statistical study was carried out with SPSS23.0. Among the studied SNPs, non-response was significantly associated with variant alleles of rs4588 (OR* = 11.51; p < 0.001), rs10766197 (OR* = 6.92; p = 0.008) and rs12794714 (OR* = 5.09; p = 0.004). These three SNPs contributed in 18.8% in response variability with rs4588 being the most influential (10.3%). There was a significant linear negative correlation between baseline 25(OH)D and post supplementation 25(OH)D concentration (r = −0.437; p < 0.001) as well as a linear negative association between the increase in 25(OH)D concentration and GRS (GRS: genetic risk score = the sum of risk alleles) (r = −0.149; p = 0.033). DBP-rs4588, CYP2R1-rs10766197 and rs12794714 variants are associated with variations in serum 25(OH)D concentrations and efficacy of response to vitD supplementation in Tunisian adults. Taking into account these variations can help to better adapt vitD intake to ensure a higher response to supplementation.
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影响因子：
20.3
作者：
Bouillon R;Marcocci C;Carmeliet G;Bikle D;White JH;Dawson-Hughes B;Lips P;Munns CF;Lazaretti-Castro M;Giustina A;Bilezikian J
通讯作者：
Bilezikian J
影响因子：
5.4
作者：
Nimitphong H;Saetung S;Chanprasertyotin S;Chailurkit LO;Ongphiphadhanakul B
通讯作者：
Ongphiphadhanakul B
影响因子：
5.8
作者：
Barry, Elizabeth L.;Rees, Judy R.;Baron, John A.
通讯作者：
Baron, John A.
影响因子：
3
作者：
Mehramiz, Mehrane;Khayyatzadeh, Sayyed Saeid;Avan, Amir
通讯作者：
Avan, Amir
影响因子：
3.2
作者：
Pott-Junior, Henrique;Luzeiro, Camila;Castelo, Adauto
通讯作者：
Castelo, Adauto