Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy.
Polymer-capped fiber-optic Raman probe for non-invasive Raman spectroscopy.
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DOI：
10.1039/c1an15847c
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发表时间：
2012-01-07
期刊：
影响因子：
--
通讯作者：
Morris MD
中科院分区：
文献类型：
--
作者：
Okagbare PI;Morris MD
光纤探头设计的进步正在推动拉曼光谱进入临床，尽管仍然存在重要的实际问题。虽然已经致力于最小化来自光纤的拉曼和荧光背景，但对产生能够校正组织反照率变化的参考拉曼信号的需要关注较少，这对于量化组织成分的变化是重要的。为了解决这一缺点，我们开发了一种光纤探头，在每根激励光纤的末端都安装了一个氟化乙丙橡胶(FEP)帽。激光通过透明帽的传输产生一个732厘米的−1拉曼频带，其强度与传输到感兴趣组织的激光功率成线性关系。在我们的第一个设计中，FEP帽起到了波导的作用，只有很小的插入损耗(~5%)。通过1 mm聚合物的激光透射率足以产生可用的参考拉曼信号。我们以大鼠腿部模型为模型，展示了该探针在动物组织定量非侵入性拉曼光谱中的应用。大鼠离体胫骨的体外拉曼光谱支持使用该探针对各种组织进行光谱分析。这些结果为拉曼探针可用于多种光谱应用提供了原理证据。
Advances in fiber optic probe design are moving Raman spectroscopy into the clinic, although there remain important practical problems. While much effort has been devoted to minimizing Raman and fluorescence background from fibers, less attention has been given to the need to generate reference Raman signals that can correct for variations in tissue albedo, which is important in quantifying changes in tissue composition. To address this shortcoming, we have developed a fiber optic probe that incorporates a fluorinated ethylene-propylene copolymer (FEP) cap at the end of each excitation fiber. Transmission of laser light through the transparent cap generates a 732 cm−1 Raman band whose intensity scales linearly with the laser power delivered to the tissue of interest. In our first design, the FEP cap functions as a waveguide with only a small insertion loss (~5%). Laser transmission through 1 mm of the polymer is sufficient to generate a usable reference Raman signal. We show the application of the probe to quantitative non-invasive Raman spectroscopy of animal tissues using rat leg phantoms as models. Ex-vivo Raman spectroscopy of excised rat tibia supports the use of the probe for spectroscopy of various tissues. These results provide proof of principle that the Raman probe can be used in multiple spectroscopic applications.
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影响因子：
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