The balanced microenvironment regulated by the degradants of appropriate PLGA scaffolds and chitosan conduit promotes peripheral nerve regeneration.
The balanced microenvironment regulated by the degradants of appropriate PLGA scaffolds and chitosan conduit promotes peripheral nerve regeneration.
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适当的PLGA支架和壳聚糖导管的降解物调节的平衡微环境促进周围神经再生
DOI：
10.1016/j.mtbio.2021.100158
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发表时间：
2021-09
期刊：
影响因子：
--
通讯作者：
Wang H
中科院分区：
文献类型：
--
作者：
Lu P;Wang G;Qian T;Cai X;Zhang P;Li M;Shen Y;Xue C;Wang H
组织工程神经移植是修复远端周围神经缺损最有前途的方法。壳聚糖和聚乳酸-羟基乙酸（PLGA）支架材料被认为是在制药和生物医学领域尤其是组织工程领域具有广阔应用前景的材料。为了进一步研究不同数量聚乳酸-羟基乙酸（PLGA）支架置入壳聚糖导管及其降解物对周围神经再生的影响，采用不同数量聚乳酸-羟基乙酸支架置入壳聚糖神经导管修复大鼠坐骨神经缺损。动态综合评价不同时间点周围神经的再生情况。此外，我们还揭示了不同剂量PLGA支架对再生微环境的影响，包括炎症反应和细胞状态。适度丰富的PLGA对神经再生的成功更有帮助，这在再生神经的结构、目标肌肉的再神经支配、神经冲动传导和整体功能方面得到了证明。PLGA支架有助于雪旺细胞的迁移和成熟。此外，PLGA和壳聚糖的降解产物以正确的比例中和，减少了炎症反应，增强了再生微环境。适当的PLGA支架降解物和壳聚糖导管调节平衡的微环境，促进周围神经再生。这一发现代表着对teng结构的编程、聚酯材料在再生医学中的应用以及对神经再生微环境的理解又向前迈进了一步。为了促进雪旺细胞的迁移和成熟，引导支架的构建是必不可少的。PLGA支架的大量酸降解产物对细胞增殖、迁移和凋亡产生不利影响。适量的PLGA支架可以平衡细胞正向引导和负向降解炎症。PLGA用量及其与互补生物材料的配合是影响再生效果的关键因素。
Tissue-engineered nerve grafts (TENGs) are the most promising way for repairing long-distance peripheral nerve defects. Chitosan and poly (lactic-co-glycolic acid) (PLGA) scaffolds are considered as the promising materials in the pharmaceutical and biomedical fields especially in the field of tissue engineering. To further clarify the effects of a chitosan conduit inserted with various quantity of poly (lactic-co-glycolic acid) (PLGA) scaffolds, and their degrades on the peripheral nerve regeneration, the chitosan nerve conduit inserted with different amounts of PLGA scaffolds were used to repair rat sciatic nerve defects. The peripheral nerve regeneration at the different time points was dynamically and comprehensively evaluated. Moreover, the influence of different amounts of PLGA scaffolds on the regeneration microenvironment including inflammatory response and cell state were also revealed. The modest abundance of PLGA is more instrumental to the success of nerve regeneration, which is demonstrated in terms of the structure of the regenerated nerve, reinnervation of the target muscle, nerve impulse conduction, and overall function. The PLGA scaffolds aid the migration and maturation of Schwann cells. Furthermore, the PLGA and chitosan degradation products in a correct ratio neutralize, reducing the inflammatory response and enhancing the regeneration microenvironment. The balanced microenvironment regulated by the degradants of appropriate PLGA scaffolds and chitosan conduit promotes peripheral nerve regeneration. The findings represent a further step towards programming TENGs construction, applying polyester materials in regenerative medicine, and understanding the neural regeneration microenvironment. Guide scaffolds are necessary for construction of TENGs to benefeat Schwann cell migration and maturation. A large number of acid degradation products of PLGA scaffolds adversely affect cell proliferation, migration and apoptosis. Appropriate amount of PLGA scaffolds balance positive cell guidance and negative degradation inflammation. Dosage of PLGA and its combination with complementary biomaterials are key factors that affect regeneration effects.
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影响因子：
16.2
作者：
Clements MP;Byrne E;Camarillo Guerrero LF;Cattin AL;Zakka L;Ashraf A;Burden JJ;Khadayate S;Lloyd AC;Marguerat S;Parrinello S
通讯作者：
Parrinello S
影响因子：
5.7
作者：
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影响因子：
5.1
作者：
Bucan V;Vaslaitis D;Peck CT;Strauß S;Vogt PM;Radtke C
通讯作者：
Radtke C
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影响因子：
2.7
作者：
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