Head-free eye tracking, and efficient receptive field mapping in the marmoset

Head-free eye tracking, and efficient receptive field mapping in the marmoset
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狨猴的无头眼动追踪和高效感受野映射

DOI:
10.1101/2020.10.30.361238
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发表时间:
2020
期刊:
bioRxiv
影响因子:
--
通讯作者:
Rohenkohl
Rohenkohl
中科院分区:
--
文献类型:
--
作者:
Jendritza;Rohenkohl

文献摘要

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绒猴已成为一个有前途的灵长类动物模型系统,特别是视觉神经科学。许多常见的实验范式依赖于头部固定和眼睛固定在显着的视觉刺激的演示期间延长的时间。这两种行为要求对绒猴来说都是挑战。在这里,我们提出了两种方法的发展,每个解决这些困难之一。首先,我们表明,这是可能的,使用一个标准的眼睛跟踪系统,没有头部固定,以评估在绒猴的视觉行为。无头动物的眼动跟踪质量足以从视觉敏锐度任务中获得精确的心理测量功能。其次,我们介绍了一种新的方法,有效的感受野(RF)映射,不依赖于移动的刺激,但使用快速闪烁的环带和楔形。我们目前的数据记录在头固定在V1和V6区,并表明,RF位置很容易在很短的记录时间内获得。因此,在这项工作中提出的方法的进步将有助于建立作为一个有价值的模型在神经科学的绒猴。
The marmoset has emerged as a promising primate model system, in particular for visual neuroscience. Many common experimental paradigms rely on head fixation and an extended period of eye fixation during the presentation of salient visual stimuli. Both of these behavioral requirements can be challenging for marmosets. Here, we present two methodological developments, each addressing one of these difficulties. First, we show that it is possible to use a standard eye-tracking system without head fixation to assess visual behavior in the marmoset. Eye-tracking quality from head-free animals is sufficient to obtain precise psychometric functions from a visual acuity task. Second, we introduce a novel method for efficient receptive field (RF) mapping that does not rely on moving stimuli but uses fast flashing annuli and wedges. We present data recorded during head-fixation in areas V1 and V6 and show that RF locations are readily obtained within a short period of recording time. Thus, the methodological advancements presented in this work will contribute to establish the marmoset as a valuable model in neuroscience.
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