The evolution and possible role of two Sox8 genes during sex differentiation in Japanese flounder (Paralichthys olivaceus)
The evolution and possible role of two Sox8 genes during sex differentiation in Japanese flounder (Paralichthys olivaceus)
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两个 Sox8 基因在日本牙鲆（Paralichthys olivaceus）性别分化过程中的进化和可能的作用
DOI：
10.1002/mrd.23136
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发表时间：
2019-05
影响因子：
2.5
通讯作者：
Yu Haiyang
中科院分区：
文献类型：
--
作者：
Yu Haiyang;Wang Yujue;Li Xiaojing;Ni Feifei;Sun Minmin;Zhang Quanqi;Wang Xubo;Yu Haiyang
Sox 8基因作为Sox家族的成员，在哺乳动物中已被广泛研究。然而，在硬骨鱼中sox 8基因的调控很少被研究，这些基因在硬骨鱼中的功能分析很少进行。在此，在日本比目鱼中鉴定了两个重复的sox 8基因，Posox 8a和Posox 8b。表达分析表明，Posox 8a和Posox 8b在睾丸支持细胞中表达，在睾丸发育和功能维持中起重要作用。正选择和系统发育分析发现，Posox 8a和Posox 8b在进化过程中都经历了纯化选择，其中sox 8最有可能是sox 8a的祖先。这些结果表明，Posox 8a和Posox 8b在日本比目鱼的三轮全基因组复制后都具有重要的生物学功能。使用细胞转染和双荧光素酶报告基因测定验证了Posox 8a和Posox 8b的功能分化; Posox 8a过表达促进了3β-羟基类固醇脱氢酶表达，Posox 8b过表达促进了细胞色素P450芳香酶（cyp 19 a1; P450 arom）表达。最后，结合Posox 8a和Posox 8b在孵化后30 ~ 100 d的表达分析，推测Posox 8a和Posox 8b可能通过调控性激素的合成参与了牙鲆的性分化和性腺发育过程。本研究首次阐明了Posox 8a和Posox 8b在比目鱼性别分化和性腺发育中的可能作用机制，为研究sox 8基因在硬骨鱼类中的功能奠定了基础。
Sox8 genes, as members of the Sox family, have been studied widely in mammals. However, regulation of sox8 genes in teleosts has rarely been studied, and functional analysis of these genes in teleosts has rarely been performed. Here, two duplicates of sox8 genes were identified in Japanese flounder, Posox8a and Posox8b. The analysis of expression showed that Posox8a and Posox8b were expressed in Sertoli cells of the testis, indicating that they play important roles in development and functional maintenance of the testis. Positive selection and phylogenetic analysis found that both Posox8a and Posox8b underwent the purification selection during evolutionary and that sox8 was most likely to be the ancestor sox8a. These results suggested that both Posox8a and Posox8b had important biological functions after generation from three rounds of whole‐genome duplication in Japanese flounder. The functional differentiation of Posox8a and Posox8b was verified using cell transfection and dual‐luciferase reporter assays; Posox8a overexpression‐promoted 3β‐hydroxysteroid dehydrogenase expression and Posox8b overexpression‐promoted cytochrome P450 aromatase (cyp19a1; P450arom) expression. Finally, combined with Posox8a and Posox8b expression analysis from 30 to 100 days after hatch, we speculated that Posox8a and Posox8b might participate in the process of sex differentiation and gonadogenesis by regulating sex hormone biosynthesis in the Japanese flounder. Our study is the first to demonstrate the possible mechanism of Posox8a and Posox8b in Japanese flounder sex differentiation and gonadogenesis, laying a solid foundation for functional studies of sox8 genes in teleosts.
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