The pyruvate dehydrogenase kinase 2 (PDK2) is associated with conidiation, mycelial growth, and pathogenicity in Fusarium graminearum
The pyruvate dehydrogenase kinase 2 (PDK2) is associated with conidiation, mycelial growth, and pathogenicity in Fusarium graminearum
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丙酮酸脱氢酶激酶 2 (PDK2) 与禾谷镰刀菌的分生孢子形成、菌丝体生长和致病性相关
DOI：
10.1186/s43014-020-00025-x
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发表时间：
2020-05
期刊：
影响因子：
--
通讯作者：
Jianrong Shi
中科院分区：
文献类型：
--
作者：
Tao Gao;Dan He;Xin Liu;Fang Ji;Jianhong Xu;Jianrong Shi
摘要：丙酮酸脱氢酶激酶（PDK）是一种存在于多种真核生物中的线粒体酶，包括植物病原体禾谷镰刀菌（Fusarium graminearum）。该酶可通过磷酸化减少葡萄糖氧化为乙酰辅酶A，并选择性地抑制丙酮酸脱氢酶（PDH）的活性，该酶是一种丙酮酸脱氢酶复合物（PDC）。在这项研究中，我们调查了F。由FgPDK 2编码的禾谷镰刀菌丙酮酸脱氢酶激酶，其是粗糙脉孢菌PDK 2的同源物。FgPDK 2基因的破坏导致了几种表型缺陷，包括对菌丝生长、分生孢子、色素沉着和致病性的影响。突变体还表现出降低的耐渗透胁迫和细胞膜/壁损伤剂。FgPDK 2缺失突变体表现出降低的毒力。所有这些缺陷都通过突变体与完整FgPDK 2基因的遗传互补来恢复。总之，结果表明FgPDK 2对F.禾谷镰刀菌赤霉病的分子生物学特性，可作为新型杀菌剂防治赤霉病的潜在分子靶标。禾本科..图形摘要
Abstract.Pyruvate dehydrogenase kinase (PDK) is a mitochondrial enzyme in a variety of eukaryotes, including the plant pathogen Fusarium graminearum. This enzyme can reduce the oxidation of glucose to acetyl-coA by phosphorylation and selectively inhibits the activity of pyruvate dehydrogenase (PDH), which is a kind of pyruvate dehydrogenase complex (PDC). In this study, we investigated the F. graminearum pyruvate dehydrogenase kinase encoded by FgPDK2, which is a homologue of Neurospora crassa PDK2. The disruption of the FgPDK2 gene led to several phenotypic defects including effects on mycelial growth, conidiation, pigmentation, and pathogenicity. The mutants also showed decreased resistance to osmotic stress and cell membrane/wall-damaging agents. The FgPDK2 deletion mutant exhibited reduced virulence. All of these defects were restored by genetic complementation of the mutant with the complete FgPDK2 gene. Overall, the results demonstrated that FgPDK2 is crucial for the growth of F. graminearum and can be exploited as a potential molecular target for novel fungicides to control Fusarium head blight caused by F. graminearum..Graphical abstract
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影响因子：
6.4
作者：
Adamo GM;Brocca S;Passolunghi S;Salvato B;Lotti M
通讯作者：
Lotti M
DOI：
10.1016/s0021-9258(18)64656-3
发表时间：
2003
期刊：
--
影响因子：
--
作者：
O. Reiss
通讯作者：
O. Reiss
影响因子：
3.2
作者：
Yu Zhang;P. Fan;X. Zhang;Changjun Chen;Mingguo Zhou
通讯作者：
Yu Zhang;P. Fan;X. Zhang;Changjun Chen;Mingguo Zhou
影响因子：
6.4
作者：
Papagianni M
通讯作者：
Papagianni M
影响因子：
2.6
作者：
J. Pronk;H. Yde Steensma;J. P. van Dijken
通讯作者：
J. Pronk;H. Yde Steensma;J. P. van Dijken