The submerged printing of cells onto a modified surface using a continuous flow microspotter.
The submerged printing of cells onto a modified surface using a continuous flow microspotter.
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DOI：
10.3791/51273
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发表时间：
2014-04-22
期刊：
影响因子：
--
通讯作者：
Brooks BD
中科院分区：
文献类型：
--
作者：
Davidoff SN;Miles AR;Romanov V;Gale BK;Eckman JW;Brooks BD
这种3D微流体打印技术将细胞阵列打印到浸没的表面上。在这里，我们描述了如何在3D流动池中将细胞阵列微流体地递送到浸没的表面上。通过在浸没的表面上打印，产生了细胞微阵列，其允许在包括癌症、糖尿病、炎症、感染和心血管疾病在内的多个领域进行药物筛选和细胞毒性评估。用于微阵列应用的细胞打印具有显著的挑战，包括在打印后维持生理相关细胞表型的问题、所需细胞的不良组织/分布以及不能将药物和/或营养物递送到阵列中的靶向区域。我们的3D微流体打印技术能够以自动化和多路复用的方式将细胞阵列密封和打印到浸没的表面上。微流控单元阵列（MFCA）3D射流的设计使打印头尖端能够下降到充满液体的井或盘中，并压靠在表面上形成密封。打印头的软硅胶尖端表现得像一个垫圈，能够通过施加压力或后退形成可逆密封。其他细胞打印技术，如针式或喷墨打印机无法在浸没应用中打印。浸没式表面打印对于保持细胞的表型和监测表面上的这些细胞而不干扰材料表面特性是必不可少的。通过在浸没的表面上打印，产生细胞微阵列，允许在包括癌症、糖尿病、炎症、感染和心血管疾病在内的多个领域进行药物筛选和细胞毒性评估。
This 3D microfluidic printing technology prints arrays of cells onto submerged surfaces. Here we describe how array of cells are delivered microfluidically in 3D flow cells onto submerged surfaces. By printing onto submerged surfaces, cell microarrays were produced that allow for drug screening and cytotoxicity assessment in a multitude of areas including cancer, diabetes, inflammation, infections, and cardiovascular disease. The printing of cells for microarray applications possesses significant challenges including the problem of maintaining physiologically relevant cell phenotype after printing, poor organization/distribution of desired cells, and the inability to deliver drugs and/or nutrients to targeted areas in the array. Our 3D microfluidic printing technology is uniquely capable of sealing and printing arrays of cells onto submerged surfaces in an automated and multiplexed manner. The design of the microfluidic cell array (MFCA) 3D fluidics enables the printhead tip to be lowered into a liquid-filled well or dish and compressed against a surface to form a seal. The soft silicone tip of the printhead behaves like a gasket and is able to form a reversible seal by applying pressure or backing away. Other cells printing technologies such as pin or ink-jet printers are unable to print in submerged applications. Submerged surface printing is essential to maintain phentotypes of cells and to monitor these cells on a surface without disturbing the material surface characteristics. By printing onto submerged surfaces, cell microarrays are produced that allow for drug screening and cytotoxicity assessment in a multitude of areas including cancer, diabetes, inflammation, infections, and cardiovascular disease.
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影响因子：
17.3
作者：
Fernandes TG;Diogo MM;Clark DS;Dordick JS;Cabral JM
通讯作者：
Cabral JM
影响因子：
16.1
作者：
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通讯作者：
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影响因子：
13.1
作者：
Gao, Dan;Liu, Hongxia;Lin, Jin-Ming
通讯作者：
Lin, Jin-Ming
影响因子：
6.1
作者：
Gottwald, Eric;Giselbrecht, Stefan;Weibezahn, Karl-Friedrich
通讯作者：
Weibezahn, Karl-Friedrich
影响因子：
4.7
作者：
Xu, Feng;Celli, Jonathan;Rizvi, Imran;Moon, Sangjun;Hasan, Tayyaba;Demirci, Utkan
通讯作者：
Demirci, Utkan