Evidence for the modulation of nociception in mice by central mast cells.
Evidence for the modulation of nociception in mice by central mast cells.
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DOI：
10.1002/ejp.1086
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发表时间：
2017-11
期刊：
影响因子：
--
通讯作者：
Larson AA
中科院分区：
文献类型：
--
作者：
Kissel CL;Kovács KJ;Larson AA
神经结扎后产生的痛觉过敏在时间上和程度上与位于结扎对侧的丘脑肥大细胞的数量相对应。我们测试的可能性，肥大细胞调节伤害性中枢，类似于他们的作用在周边。我们研究了两种痛觉过敏化合物的中枢作用，这两种化合物也会诱导肥大细胞脱粒并使用克罗洛芬稳定肥大细胞。由神经生长因子（NGF，一种神经营养化合物）诱导的热痛觉过敏（甩尾）和由强啡肽A（1-17）（阿片样物质化合物）诱导的机械痛觉过敏（von Frey）各自与脱颗粒的丘脑肥大细胞的百分比相关。这些肥大细胞的脱粒通过中央注射的化合物48/80，缺乏神经营养或阿片样物质的活性，足以重演热痛觉过敏。稳定肥大细胞的中央注射crovalyn产生的基线甩尾或冯弗雷纤维敏感性没有镇痛作用，但抑制热痛觉过敏产生的化合物48/80和触觉痛觉过敏引起的强啡肽和弗氏完全佐剂。最后，由福尔马林直接激活伤害感受器（I期）产生的化学伤害感受不受中枢注射crovaln的抑制，而依赖于中枢致敏（福尔马林II期和乙酸诱导的腹部伸展）的化学伤害感受受抑制。这些证据表明，位于中央的肥大细胞的脱粒敏感中枢伤害性通路，导致痛觉过敏和紧张性化学敏感性。
Hyperalgesia that develops following nerve ligation corresponds temporally and in magnitude with the number of thalamic mast cells located contralateral to the ligature. We tested the possibility that mast cells modulate nociception centrally, similar to their role in the periphery. We examined the central effect of two hyperalgesic compounds that also induce mast cell degranulation and stabilized mast cells using cromolyn. Thermal hyperalgesia (tail flick) induced by nerve growth factor (NGF, a neurotrophic compound) and mechanical hyperalgesia (von Frey) induced by dynorphin A (1–17) (opioid compound) each correlated with the percent of thalamic mast cells that were degranulated. Degranulation of these mast cells by the central injection of compound 48/80, devoid of neurotrophic or opioid activity, was sufficient to recapitulate thermal hyperalgesia. Stabilization of mast cells by central injections of cromolyn produced no analgesic effect on baseline tail flick or von Frey fiber sensitivity, but inhibited thermal hyperalgesia produced by compound 48/80 and tactile hyperalgesia induced by dynorphin and by Freund’s complete adjuvant. Finally, chemical nociception produced by the direct activation of nociceptors by formalin (phase I) was not inhibited by centrally injected cromolyn whereas chemical nociception dependent on central sensitization (formalin-phase II and acetic acid-induced abdominal stretches) was. These convergent lines of evidence suggest that degranulation of centrally located mast cells sensitizes central nociceptive pathways leading to hyperalgesia and tonic chemical sensitivity.
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影响因子：
4.7
作者：
Abdelhamid RE;Kovacs KJ;Pasley JD;Nunez MG;Larson AA
通讯作者：
Larson AA
影响因子：
4.8
作者：
Kempuraj, D;Papadopoulou, NG;Theoharides, TC
通讯作者：
Theoharides, TC
影响因子：
--
作者：
ABERCROMBIE, M
通讯作者：
ABERCROMBIE, M
DOI：
10.1073/pnas.78.10.6543
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
CHAVKIN, C;GOLDSTEIN, A
通讯作者：
GOLDSTEIN, A
影响因子：
7.3
作者：
COLLIER, HOJ;DINNEEN, LC;SCHNEIDER, C
通讯作者：
SCHNEIDER, C