Evaluating the lethal and pre-lethal effects of a range of fungi against adult Anopheles stephensi mosquitoes.
Evaluating the lethal and pre-lethal effects of a range of fungi against adult Anopheles stephensi mosquitoes.
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评估一系列真菌对成年蚊子斯蒂芬西蚊子的致命和致命作用。
DOI：
10.1186/1475-2875-11-365
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发表时间：
2012-11-05
期刊：
影响因子：
3
通讯作者：
Thomas MB
中科院分区：
文献类型：
--
作者：
Blanford S;Jenkins NE;Read AF;Thomas MB
抗药性严重破坏了消灭疟疾的努力。作为回应，对使用化学杀虫剂对付成蚊病媒的替代品的研究一直在增加。真菌昆虫病原菌作为生物农药已受到广泛关注，并显示出相当大的潜力。这项研究必须集中在相对较少的真菌菌株，以“证明概念”。此外，大多数注意力都集中在检查真菌毒力（致死率），而不是真菌感染的其他特性，这些特性也可能有助于降低传播潜力。在这里，筛选了一系列真菌分离株，以检查毒力的变化以及这与摄食倾向的额外致死前减少的关系。将亚洲疟疾媒介斯氏按蚊暴露于属于球孢白僵菌、金龟子绿僵菌、嗜杀绿僵菌和粉棒束孢菌的17种不同昆虫病原真菌分离株。将每种分离株以1×109孢子ml-1的标准剂量率应用于测试基质，并将蚊子暴露6小时。随后，将昆虫移到网笼中，在接下来的14天内监测存活率。在该孵育期间，通过在笼的侧面提供摄食刺激物并记录探测的数量来测定每种分离物的蚊子的摄食倾向。真菌分离株对A. stephensi，其中一些在7天内引起>80%的死亡率，而其他的在研究期间相对于对照引起死亡率几乎没有增加。类似地，一些分离株对摄食倾向有很大影响，导致摄食率在致死前减少>50%，而其他分离株的影响很小。真菌致病力与减食率之间存在明显的相关性，致病力可以解释减食率变化的近70%。然而，也有一些菌株，无论是喂养下降与高毒力，或毒力并没有自动提示喂养大幅度下降。这些结果进行了讨论的背景下，选择最佳的真菌菌株的生物农药的发展。
Insecticide resistance is seriously undermining efforts to eliminate malaria. In response, research on alternatives to the use of chemical insecticides against adult mosquito vectors has been increasing. Fungal entomopathogens formulated as biopesticides have received much attention and have shown considerable potential. This research has necessarily focused on relatively few fungal isolates in order to ‘prove concept’. Further, most attention has been paid to examining fungal virulence (lethality) and not the other properties of fungal infection that might also contribute to reducing transmission potential. Here, a range of fungal isolates were screened to examine variation in virulence and how this relates to additional pre-lethal reductions in feeding propensity. The Asian malaria vector, Anopheles stephensi was exposed to 17 different isolates of entomopathogenic fungi belonging to species of Beauveria bassiana, Metarhizium anisopliae, Metarhizium acridum and Isaria farinosus. Each isolate was applied to a test substrate at a standard dose rate of 1×109 spores ml-1 and the mosquitoes exposed for six hours. Subsequently the insects were removed to mesh cages where survival was monitored over the next 14 days. During this incubation period the mosquitoes’ propensity to feed was assayed for each isolate by offering a feeding stimulant at the side of the cage and recording the number probing. Fungal isolates showed a range of virulence to A. stephensi with some causing >80% mortality within 7 days, while others caused little increase in mortality relative to controls over the study period. Similarly, some isolates had a large impact on feeding propensity, causing >50% pre-lethal reductions in feeding rate, whereas other isolates had very little impact. There was clear correlation between fungal virulence and feeding reduction with virulence explaining nearly 70% of the variation in feeding reduction. However, there were some isolates where either feeding decline was not associated with high virulence, or virulence did not automatically prompt large declines in feeding. These results are discussed in the context of choosing optimum fungal isolates for biopesticide development.
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影响因子：
11.8
作者：
Asidi A;N'Guessan R;Akogbeto M;Curtis C;Rowland M
通讯作者：
Rowland M
DOI：
10.1177/1534582306289580
发表时间：
2006-09-01
期刊：
Behavioral and cognitive neuroscience reviews
影响因子：
--
作者：
Adamo, Shelley A
通讯作者：
Adamo, Shelley A
影响因子：
1.4
作者：
Darbro, Jonathan M.;Graham, Robert I.;Thomas, Matthew B.
通讯作者：
Thomas, Matthew B.
DOI：
10.4269/ajtmh.2008.78.910
发表时间：
2008-06-01
影响因子：
3.3
作者：
Farenhorst, Marit;Farina, Daniela;Knols, Bart G. J.
通讯作者：
Knols, Bart G. J.
DOI：
10.1098/rspb.2008.0689
发表时间：
2009-01-07
影响因子：
4.7
作者：
Hancock, P. A.;Thomas, M. B.;Godfray, H. C. J.
通讯作者：
Godfray, H. C. J.