FON2 SPARE1 redundantly regulates floral meristem maintenance with FLORAL ORGAN NUMBER2 in rice.
FON2 SPARE1 redundantly regulates floral meristem maintenance with FLORAL ORGAN NUMBER2 in rice.
复制标题
DOI：
10.1371/journal.pgen.1000693
复制
发表时间：
2009-10
期刊：
影响因子：
4.5
通讯作者：
Hirano HY
中科院分区：
文献类型：
--
作者：
Suzaki T;Ohneda M;Toriba T;Yoshida A;Hirano HY
CLAVATA 信号传导限制拟南芥茎尖分生组织 (SAM) 中的干细胞身份。在水稻 (Oryza sativa) 中，花器官 NUMBER2 (FON2) 与 CLV3 密切相关，作为信号分子参与类似途径，负调控花分生组织 (FM) 中的干细胞增殖。在这里，我们展示了编码 CLE 蛋白的 FON2 SPARE1 (FOS1) 基因与 FON2 一起在维持 FM 中发挥作用。此外，FOS1 似乎参与了营养期 SAM 的维持，因为 FOS1 的组成型表达导致了营养期 SAM 的终止。遗传分析表明，FOS1 的作用不需要 FON1（FON2 的假定受体），这表明 FOS1 和 FON2 可能作为独立途径中的信号分子在分生组织维持中发挥作用。最初，我们将 FOS1 鉴定为源自 O. sativa indica 的抑制子，并抑制 O. sativa japonica 中的 fon2 突变。粳稻中的 FOS1 功能似乎受到信号肽假定加工位点的功能性核苷酸多态性 (FNP) 的影响。对大约 150 个驯化稻和野生稻物种中 FOS1 的序列比较表明，这种 FNP 仅存在于粳稻中，这表明 FOS1 和 FON2 的冗余调控在稻属物种中很常见。 FNP 的分布还表明，这种突变可能发生在粳稻与其野生祖先分化期间。水稻中干细胞的维持可能受到至少三种负向途径的调节，并且根据分生组织的类型，每种途径对这种调节的贡献可能不同。这种情况与拟南芥中的情况形成鲜明对比，在拟南芥中，CLV 信号传导是所有分生组织中的主要单一途径。植物的身体规划由胚胎中产生的顶端分生组织的功能调节。叶和花器官分别源自营养芽顶端分生组织（SAM）和花分生组织（FM）中的干细胞提供的细胞。因此，干细胞维持的遗传调控是植物发育的中心问题。在模型植物拟南芥中，CLAVATA3 (CLV3) 作为关键信号分子来限制 SAM 和 FM 中干细胞群的大小。然而，在水稻中，我们发现两个类似 CLV3 的基因，花器官编号 2 (FON2) 和 FON2 SPARE1 (FOS1)，冗余地调节 FM 的维持。我们还表明，FOS1 可能参与营养 SAM 的维持，而 FON2 在此分生组织的调节中不起任何作用。 FOS1 似乎通过与 FON2 受体不同的假定受体发挥作用，表明这两种信号分子在独立的途径中发挥作用，根据分生组织的类型以不同的方式限制干细胞。此外，我们还发现标准水稻 Oryza sativa spp 中的 FOS1 基因受到损害。粳稻，在水稻的进化过程中。
CLAVATA signaling restricts stem cell identity in the shoot apical meristem (SAM) in Arabidopsis thaliana. In rice (Oryza sativa), FLORAL ORGAN NUMBER2 (FON2), closely related to CLV3, is involved as a signaling molecule in a similar pathway to negatively regulate stem cell proliferation in the floral meristem (FM). Here we show that the FON2 SPARE1 (FOS1) gene encoding a CLE protein functions along with FON2 in maintenance of the FM. In addition, FOS1 appears to be involved in maintenance of the SAM in the vegetative phase, because constitutive expression of FOS1 caused termination of the vegetative SAM. Genetic analysis revealed that FOS1 does not need FON1, the putative receptor of FON2, for its action, suggesting that FOS1 and FON2 may function in meristem maintenance as signaling molecules in independent pathways. Initially, we identified FOS1 as a suppressor that originates from O. sativa indica and suppresses the fon2 mutation in O. sativa japonica. FOS1 function in japonica appears to be compromised by a functional nucleotide polymorphism (FNP) at the putative processing site of the signal peptide. Sequence comparison of FOS1 in about 150 domesticated rice and wild rice species indicates that this FNP is present only in japonica, suggesting that redundant regulation by FOS1 and FON2 is commonplace in species in the Oryza genus. Distribution of the FNP also suggests that this mutation may have occurred during the divergence of japonica from its wild ancestor. Stem cell maintenance may be regulated by at least three negative pathways in rice, and each pathway may contribute differently to this regulation depending on the type of the meristem. This situation contrasts with that in Arabidopsis, where CLV signaling is the major single pathway in all meristems. The body plan of plants is regulated by the function of apical meristems that are generated in the embryo. Leaves and floral organs are derived from cells supplied by stem cells in the vegetative shoot apical meristem (SAM) and the floral meristem (FM), respectively. Thus, genetic regulation of stem cell maintenance is a central issue in plant development. In the model plant Arabidopsis thaliana, CLAVATA3 (CLV3) functions as a key signaling molecule to restrict the size of the stem cell population in both the SAM and the FM. In rice, however, we show here that two CLV3-like genes, FLORAL ORGAN NUMBER2 (FON2) and FON2 SPARE1 (FOS1), redundantly regulate maintenance of the FM. We also show that FOS1 is likely to be involved in maintenance of the vegetative SAM, whereas FON2 plays no role in regulation in this meristem. FOS1 appears to act via a putative receptor that differs from the FON2 receptor, suggesting that these two signaling molecules function in independent pathways to restrict stem cells in different ways depending on the type of meristem. In addition, we show that the FOS1 gene was compromised in the standard rice, Oryza sativa spp. japonica, during the evolution of rice.
登录
查看更多内容
影响因子：
56.9
作者：
Fletcher, LC;Brand, U;Meyerowitz, EM
通讯作者：
Meyerowitz, EM
影响因子：
2.4
作者：
Kojima, Y;Ebana, K;Kawase, M
通讯作者：
Kawase, M
影响因子：
4.9
作者：
Clement, M;Posada, D;Crandall, KA
通讯作者：
Crandall, KA
影响因子：
56.9
作者：
Kondo, Tatsuhiko;Sawa, Shinichiro;Sakagami, Youji
通讯作者：
Sakagami, Youji
影响因子：
11.6
作者：
Mueller, Ralf;Bleckmann, Andrea;Simon, Ruediger
通讯作者：
Simon, Ruediger