Members of the germin-like protein family in Brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of Sclerotinia sclerotiorum.
Members of the germin-like protein family in Brassica napus are candidates for the initiation of an oxidative burst that impedes pathogenesis of Sclerotinia sclerotiorum.
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DOI：
10.1093/jxb/ers203
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发表时间：
2012-09
影响因子：
6.9
通讯作者：
Cai D
中科院分区：
文献类型：
--
作者：
Rietz S;Bernsdorff FE;Cai D
发芽样蛋白（GLPs）是一种与大麦发芽蛋白序列同源的蛋白，在植物中普遍存在。相关基因的分析揭示了glp在植物发育、生物和非生物胁迫中的多种功能。本研究从甘蓝型油菜（Brassica napus, BnGLP）中鉴定出一个名为BnGLP1-BnGLP14的14个芽孢样基因家族，并研究了BnGLPs在植物防御坏死性菌核菌（Sclerotinia sclerotiorum）中的潜在功能。序列比对和系统发育分析将14个bnglp分为4类，与已知的细菌草酸氧化酶有明显区别。通过比较易感甘蓝型油菜‘猎鹰’和部分抗性甘蓝型油菜‘中爽9号’，研究了BnGLP基因对菌核病菌感染的转录反应。在所检测的14个BnGLP基因中，BnGLP3基因在感染菌核葡萄球菌后6小时在两个甘蓝型油菜品种中均出现转录上调，而BnGLP12基因的上调仅限于抗性甘蓝型油菜‘中双9’。对5个具有代表性的BnGLP成员的生化分析发现，BnGLP3和BnGLP12的高分子量复合物具有生成h2o2的超氧化物歧化酶活性。同样，6h后，甘蓝型油菜‘中爽9号’的H2O2产生量高于‘猎鹰’。相反，外源施用H2O2显著降低了甘蓝型油菜‘猎鹰’的敏感性。这些数据表明，BnGLP3和BnGLP12的早期诱导参与了氧化爆发，这可能在甘蓝型油菜防御菌核病菌中起关键作用。
Germin-like proteins (GLPs) are defined by their sequence homology to germins from barley and are present ubiquitously in plants. Analyses of corresponding genes have revealed diverse functions of GLPs in plant development and biotic and abiotic stresses. This study describes the identification of a family of 14 germin-like genes from Brassica napus (BnGLP) designated BnGLP1–BnGLP14 and investigated potential functions of BnGLPs in plant defense against the necrotrophic fungus Sclerotinia sclerotiorum. Sequence alignment and phylogenetic analyses classify the 14 BnGLPs into four groups, which were clearly distinguished from known germin oxalic acid oxidases. Transcriptional responses of the BnGLP genes to S. sclerotiorum infection was determined by comparing cultivars of susceptible B. napus ‘Falcon’ and partially resistant B. napus ‘Zhongshuang 9’. Of the 14 BnGLP genes tested, BnGLP3 was transcriptionally upregulated in both B. napus cultivars at 6h after S. sclerotiorum infection, while upregulation of BnGLP12 was restricted to resistant B. napus ‘Zhongshuang 9’. Biochemical analysis of five representative BnGLP members identified a H2O2-generating superoxide dismutase activity only for higher molecular weight complexes of BnGLP3 and BnGLP12. By analogy, H2O2 formation at infected leaf sites increased after 6h, with even higher H2O2 production in B. napus ‘Zhongshuang 9’ compared with B. napus ‘Falcon’. Conversely, exogenous application of H2O2 significantly reduced the susceptibility of B. napus ‘Falcon’. These data suggest that early induction of BnGLP3 and BnGLP12 participates in an oxidative burst that may play a pivotal role in defence of B. napus against S. sclerotiorum.
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影响因子：
5.1
作者：
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通讯作者：
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影响因子：
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