Analysis of gene alterations of mitochondrial DNA D-loop regions to determine breast cancer clonality.
Analysis of gene alterations of mitochondrial DNA D-loop regions to determine breast cancer clonality.
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DOI：
10.1038/bjc.2012.505
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发表时间：
2012-12-04
影响因子：
8.8
通讯作者：
Osamura, R. Y.
中科院分区：
文献类型：
--
作者：
Masuda, S.;Kadowaki, T.;Kumaki, N.;Tang, X.;Tokuda, Y.;Yoshimura, S.;Takekoshi, S.;Osamura, R. Y.
准确测定乳腺癌的克隆性一直是一个挑战。本研究的目的是评估使用福尔马林固定石蜡包埋（FFPE）组织的方法，以区分新的原发性肿瘤与真正的复发，与较差的复发，往往需要更积极的治疗。我们研究了分析线粒体DNA D环区基因改变的新方法（GAMARA），并将其与分析X染色体连锁的人类雄激素受体（HUMARA）的传统方法进行了比较。检查原发性和继发性乳腺癌、非肿瘤性乳腺和淋巴结的FFPE切片。HUMARA、GAMMARA和联合分析的信息率分别为42.1%、76.9%和89.5%。所有10例对侧乳腺癌均被确定为非克隆性。与此相反，8例同侧继发性肿瘤中有3例（37.5%）与原发性肿瘤具有克隆起源，并被归类为真正的复发，而8例中有4例（50%）被归类为新的原发性肿瘤。伽马射线分析代表了一种新的和有用的分子方法，用于检查原发性和继发性肿瘤的精确细胞谱系，并且在确定克隆性方面比HUMARA更准确。
It has been a challenge to determine breast cancer clonality accurately. The aim of the present study was to assess methods using formalin-fixed paraffin-embedded (FFPE) tissue to differentiate new primary tumours from true recurrences that are associated with poorer prognoses and often require more aggressive treatment. We investigated the novel method of analysing gene alterations of mitochondrial DNA D-loop region (GAMDDL) and compared it with the conventional method of analysing the X-chromosome-linked human androgen receptor (HUMARA). The FFPE sections of primary and secondary breast cancers, the non-neoplastic mammary gland, and lymph nodes were examined. Informative rates for HUMARA, GAMDDL, and combined analyses were 42.1%, 76.9%, and 89.5%, respectively. All of the 10 contralateral breast cancers were determined to be non-clonal. In contrast, 3 out of 8 (37.5%) of the ipsilateral secondary tumours shared a clonal origin with the primary tumour and were classified as true recurrences, whereas 4 out of 8 (50%) were classified as new primary tumours. GAMDDL analysis represents a novel and useful molecular method for examining the precise cell lineages of primary and secondary tumours, and was more accurate than HUMARA in determining clonality.
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影响因子：
14.5
作者：
Salk JJ;Horwitz MS
通讯作者：
Horwitz MS
DOI：
10.1158/1940-6207.capr-10-0326
发表时间：
2011-05
期刊：
Cancer prevention research (Philadelphia, Pa.)
影响因子：
--
作者：
Chatterjee A;Dasgupta S;Sidransky D
通讯作者：
Sidransky D
影响因子：
3.5
作者：
Goldstein, NS;Vicini, FA;Kestin, LL
通讯作者：
Kestin, LL
影响因子：
3.8
作者：
Kollias, J;Man, S;Brook, JD
通讯作者：
Brook, JD
影响因子：
2.1
作者：
Radpour, Ramin;Fan, Alex XiuCheng;Zhong, Xiao Yan
通讯作者：
Zhong, Xiao Yan