Impact of fibroblast activation protein on osteosarcoma cell lines in vitro.
Impact of fibroblast activation protein on osteosarcoma cell lines in vitro.
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DOI：
10.3892/ol.2014.1788
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发表时间：
2014-03
期刊：
影响因子：
2.9
通讯作者：
Jiang WG
中科院分区：
文献类型：
--
作者：
Ding L;Ye L;Xu J;Jiang WG
成纤维细胞活化蛋白（FAP）或seprase，属于II型整合丝氨酸蛋白酶，是一种整合膜丝氨酸肽酶。已有研究表明FAP对肿瘤的生长、增殖和侵袭有影响。然而，FAP在骨肉瘤（OS）中发挥的细胞功能作用仍不清楚。本研究的目的是研究FAP在OS细胞系中的活性。通过锤头状核酶转基因敲除FAP的基因表达，并使用一系列体外功能测定来测试敲除细胞与其对照细胞之间的各种功能。结果表明，FAP基因敲低后MG-63和HOS细胞的生长、黏附、迁移和侵袭能力均明显低于对照组（P<0.05）。总之，FAP影响OS细胞，并可能在OS肿瘤的进展和转移中发挥作用。
Fibroblast activation protein (FAP) or seprase, which belongs to the group type II integral serine proteases, is an integral membrane serine peptidase. Previous studies have demonstrated that FAP has an effect on tumor growth, proliferation and invasion. However, the cellular functional role that FAP plays in osteosarcoma (OS) remains unknown. The aim of the present study was to investigate the activities of FAP in OS cell lines. The gene expression of FAP was knocked down through a hammerhead ribozyme transgene, and the various functions between the knockdown cells and their control cells were tested using a series of functional assays in vitro. The results indicated that knockdown of FAP markedly reduced the ability of cellular growth, matrix adhesion, migration and invasion in MG-63 and HOS cell lines compared with the control cells (P<0.05). In conclusion, FAP influences OS cells and may play a role in OS tumor progression and metastasis.
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影响因子：
6.4
作者：
Dohi O;Ohtani H;Hatori M;Sato E;Hosaka M;Nagura H;Itoi E;Kokubun S
通讯作者：
Kokubun S
影响因子：
8.8
作者：
Jiang, W G;Hiscox, S;Hallett, M B;Scott, C;Horrobin, D F;Puntis, M C
通讯作者：
Puntis, M C
影响因子：
8
作者：
Ramirez-Montagut, T;Blachere, NE;Houghton, AN
通讯作者：
Houghton, AN
影响因子：
--
作者：
Javidroozi, Mazyar;Zucker, Stanley;Chen, Wen-Tien
通讯作者：
Chen, Wen-Tien
影响因子：
3.6
作者：
Chen, He;Yang, Wei-Wei;Chen, Ming
通讯作者：
Chen, Ming