The majority of SARS-CoV-2-specific antibodies in COVID-19 patients with obesity are autoimmune and not neutralizing.
The majority of SARS-CoV-2-specific antibodies in COVID-19 patients with obesity are autoimmune and not neutralizing.
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DOI：
10.1038/s41366-021-01016-9
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发表时间：
2022-03
期刊：
影响因子：
--
通讯作者：
Blomberg BB
中科院分区：
文献类型：
--
作者：
Frasca D;Reidy L;Romero M;Diaz A;Cray C;Kahl K;Blomberg BB
肥胖会降低COVID-19患者血液中sars - cov -2特异性IgG抗体的分泌。然而，肥胖如何影响分泌的抗体的质量尚不清楚。因此，本研究的目的是评估COVID-19肥胖患者中中和抗体与自身免疫抗体的存在。从住院和门诊收集了30份经RT-PCR检测为SARS-CoV-2感染阳性个体的血清样本。其中，15人消瘦（BMI < 25）， 15人肥胖（BMI≥30）。对照血清样本来自本次大流行之前招募的30名年龄、性别和bmi匹配的未感染个体。ELISA法检测中和抗体和自身免疫抗体。IgG自身免疫抗体对丙二醛（MDA）（氧化应激和脂质过氧化的标志物）和脂肪细胞衍生蛋白抗原（AD）（肥胖脂肪组织中病毒诱导的细胞死亡的标志物）具有特异性。SARS-CoV-2感染在所有瘦的COVID-19患者中诱导中和抗体，但仅在少数肥胖的COVID-19患者中。与未感染的对照组相比，SARS-CoV-2感染在瘦患者中也比在肥胖患者中更多地诱导抗mda和抗ad自身免疫抗体。然而，这些自身免疫抗体的血清水平在肥胖的COVID-19患者中总是高于消瘦的患者。此外，由于在COVID-19患者血清样本中发现的自身免疫性抗体与血清c反应蛋白（CRP）水平相关，c反应蛋白是炎症的一般标志物，我们还评估了抗mda和抗ad抗体与血清CRP的关系，发现CRP与自身免疫性抗体呈正相关。我们的研究结果强调了评估COVID-19肥胖患者抗体反应质量的重要性，特别是自身免疫抗体的存在，并确定自我耐受性破坏的生物标志物。这对于保护这些易受感染人群至关重要，他们对SARS-CoV-2感染的反应较差的风险高于对照组。
Obesity decreases the secretion of SARS-CoV-2-specific IgG antibodies in the blood of COVID-19 patients. How obesity impacts the quality of the antibodies secreted, however, is not understood. Therefore, the objective of this study is to evaluate the presence of neutralizing versus autoimmune antibodies in COVID-19 patients with obesity. Thirty serum samples from individuals who tested positive for SARS-CoV-2 infection by RT-PCR were collected from inpatient and outpatient settings. Of these, 15 were lean (BMI < 25) and 15 were obese (BMI ≥ 30). Control serum samples were from 30 uninfected individuals, age-, gender-, and BMI-matched, recruited before the current pandemic. Neutralizing and autoimmune antibodies were measured by ELISA. IgG autoimmune antibodies were specific for malondialdehyde (MDA), a marker of oxidative stress and lipid peroxidation, and for adipocyte-derived protein antigens (AD), markers of virus-induced cell death in the obese adipose tissue. SARS-CoV-2 infection induces neutralizing antibodies in all lean but only in few obese COVID-19 patients. SARS-CoV-2 infection also induces anti-MDA and anti-AD autoimmune antibodies more in lean than in obese patients as compared to uninfected controls. Serum levels of these autoimmune antibodies, however, are always higher in obese versus lean COVID-19 patients. Moreover, because the autoimmune antibodies found in serum samples of COVID-19 patients have been correlated with serum levels of C-reactive protein (CRP), a general marker of inflammation, we also evaluated the association of anti-MDA and anti-AD antibodies with serum CRP and found a positive association between CRP and autoimmune antibodies. Our results highlight the importance of evaluating the quality of the antibody response in COVID-19 patients with obesity, particularly the presence of autoimmune antibodies, and identify biomarkers of self-tolerance breakdown. This is crucial to protect this vulnerable population at higher risk of responding poorly to infection with SARS-CoV-2 than lean controls.
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影响因子：
4.6
作者：
Lin YS;Lin CF;Fang YT;Kuo YM;Liao PC;Yeh TM;Hwa KY;Shieh CC;Yen JH;Wang HJ;Su IJ;Lei HY
通讯作者：
Lei HY
影响因子：
9
作者：
Newton AH;Cardani A;Braciale TJ
通讯作者：
Braciale TJ
DOI：
10.1146/annurev-cellbio-011620-034148
发表时间：
2020-10-06
影响因子：
11.3
作者：
Frasca D;Diaz A;Romero M;Garcia D;Blomberg BB
通讯作者：
Blomberg BB
影响因子：
4.9
作者：
O'Shea, D.;Corrigan, M.;Hogan, A. E.
通讯作者：
Hogan, A. E.
影响因子：
3.7
作者：
Frasca D;Reidy L;Cray C;Diaz A;Romero M;Kahl K;Blomberg BB
通讯作者：
Blomberg BB