Cell adhesion of Shewanella oneidensis to iron oxide minerals: Effect of different single crystal faces.

Cell adhesion of Shewanella oneidensis to iron oxide minerals: Effect of different single crystal faces.
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DOI:
10.1186/1467-4866-6-77
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发表时间:
2005
影响因子:
2.3
通讯作者:
--
中科院分区:
地球科学3区
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--
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实验结果旨在测试的假设,近表面的氧化铁矿物的分子结构影响的异化铁还原菌的粘附。这些实验涉及的测量,使用原子力显微镜,希瓦氏菌oneidensis MR-1细胞和氧化铁矿物的单晶生长面之间产生的相互作用力。细胞与赤铁矿(001)面、磁铁矿(100)面和(111)面的粘附力有显著差异。静电相互作用的作用是显而易见的。在矿物表面产生的相对粘附力的趋势是在协议与预测的铁网站密度先前公布的。这些结果表明,近表面结构确实影响初始细胞附着到氧化铁表面,这是否是通过特定的细胞表面矿物表面相互作用或更一般的界面现象介导的仍然未经测试。
The results of experiments designed to test the hypothesis that near-surface molecular structure of iron oxide minerals influences adhesion of dissimilatory iron reducing bacteria are presented. These experiments involved the measurement, using atomic force microscopy, of interaction forces generated between Shewanella oneidensis MR-1 cells and single crystal growth faces of iron oxide minerals. Significantly different adhesive force was measured between cells and the (001) face of hematite, and the (100) and (111) faces of magnetite. A role for electrostatic interactions is apparent. The trend in relative forces of adhesion generated at the mineral surfaces is in agreement with predicted ferric site densities published previously. These results suggest that near-surface structure does indeed influence initial cell attachment to iron oxide surfaces; whether this is mediated via specific cell surface-mineral surface interactions or by more general interfacial phenomena remains untested.
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