A bioactive 60-kilodalton prolactin species is preferentially secreted in cultures of mitogen-stimulated and nonstimulated peripheral blood mononuclear cells from subjects with systemic lupus erythematosus.
A bioactive 60-kilodalton prolactin species is preferentially secreted in cultures of mitogen-stimulated and nonstimulated peripheral blood mononuclear cells from subjects with systemic lupus erythematosus.
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系统性红斑狼疮患者的有丝分裂原刺激和未刺激的外周血单核细胞培养物中优先分泌具有生物活性的 60 千道尔顿催乳素。
DOI：
10.1210/jcem.82.11.4356
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发表时间：
1997
期刊：
影响因子：
--
通讯作者：
Donato Alarcón
中科院分区：
文献类型：
--
作者：
Fernando Larrea;Araceli Martı́nez;Vı́ctor Cabrera;Jorge Alcocer;Gloria Queipo;Cecilia Cariño;Donato Alarcón
我们评估了正常人和系统性红斑狼疮（SLE）患者外周血单个核细胞（PBMNC）产生PRL的能力。从基础和Con-A刺激的PBMNC制备的条件培养基通过其刺激PRL反应性Nb 2大鼠淋巴瘤细胞生长的能力来评估PRL样的存在。在Con-A存在或不存在的情况下，SLE PBMNC分泌的具有生物活性的PRL样物质的量显著高于（P < 0.001）正常细胞。用抗人PRL的特异性抗血清抑制Nb 2细胞在条件培养基中的生长。使用多克隆抗体的人PRL蛋白质印迹法显示一个单一的60 kDa的PRL样物质在正常和SLE PBMNC提取物，免疫反应性的优先发现在SLE受试者。用逆转录-PCR技术扩增出一条633 bp的特异性条带，并以人PRL互补DNA为探针进行Southern印迹分析，进一步证实了其与垂体PRL的相似性。我们的结论是高分子量的PRL样物质的合成和分泌的PBMNC，SLE患者有淋巴细胞衍生的PRL样物质的分泌增加。
We have evaluated the production of PRL by human peripheral mononuclear cells (PBMNC) from normal subjects and patients with systemic lupus erythematosus (SLE). Conditioned medium prepared from basal and Con-A-stimulated PBMNC was assessed for the presence of PRL-like by its ability to stimulate growth of PRL-responsive Nb2 rat lymphoma cells. In the presence or absence of Con-A, SLE PBMNC secrete significantly higher (P < 0.001) amounts of bioactive PRL-like species than normal cells. Growth of Nb2 cells by conditioned medium was inhibited with specific antiserum to human PRL. Western blotting using a polyclonal antibody to human PRL revealed a single 60-kDa PRL-like species in both normal and SLE PBMNC extracts, the immunoreactivity of which was preferentially found in SLE subjects. With the use of reverse transcription-PCR an expected 633-bp band was observed, and its similarity to pituitary PRL was further confirmed by Southern blot analysis with human PRL complementary DNA as a probe. We conclude that a high molecular mass PRL-like species is synthesized and secreted by PBMNC, and patients with SLE have an increased secretion of lymphocyte-derived PRL-like material.
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大鼠 Nb 2 淋巴瘤细胞的激活和抑制机制：催乳素与免疫系统之间相互作用的模型。
DOI：
10.1006/excr.1995.1192
发表时间：
1995
期刊：
Experimental cell research.
影响因子：
--
作者：
Bates,LG;Grove,DS;Mastro,AM
通讯作者：
Mastro,AM
刀豆球蛋白 A 刺激的小鼠脾细胞产生具有催乳素样生物活性和免疫反应性的因子。
DOI：
10.1016/0006-291x(87)91020-5
发表时间：
1987
影响因子：
3.1
作者：
Montgomery,DW;Zukoski,CF;Shah,GN;Buckley,AR;Pacholczyk,T;Russell,DH
通讯作者：
Russell,DH
影响因子：
--
作者：
Petri,M;Howard,D;Repke,J
通讯作者：
Repke,J
正常鼠淋巴细胞合成的催乳素样蛋白的鉴定。
影响因子：
4.8
作者：
Montgomery,DW;LeFevre,JA;Ulrich,ED;Adamson,CR;Zukoski,CF
通讯作者：
Zukoski,CF
DOI：
10.1530/acta.0.1250067
发表时间：
1991-07-01
期刊：
ACTA ENDOCRINOLOGICA
影响因子：
--
作者：
ESQUIFINO, AI;VILLANUA, MA;BARTKE, A
通讯作者：
BARTKE, A