Photocontrolled miR-148b nanoparticles cause apoptosis, inflammation and regression of Ras induced epidermal squamous cell carcinomas in mice.
Photocontrolled miR-148b nanoparticles cause apoptosis, inflammation and regression of Ras induced epidermal squamous cell carcinomas in mice.
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DOI：
10.1016/j.biomaterials.2020.120212
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发表时间：
2020-10
期刊：
影响因子：
14
通讯作者：
Hayes DJ
中科院分区：
文献类型：
--
作者：
Liu Y;Bailey JT;Abu-Laban M;Li S;Chen C;Glick AB;Hayes DJ
Despite evidence that microRNAs (miRNAs) are essential in modulating tumorigenesis, a major challenge in cancer treatment is to achieve tumor-specific selectivity and efficient yet safe delivery of miRNAs in vivo. In this study, we have developed a light-inducible silver nanoparticle nucleic acid delivery system that demonstrates precise spatiotemporal control, high cellular uptake, low cytotoxicity, escape from endosomes and release of functional miRNA into the cytosol. Using this approach, we delivered exogenous miR-148b to induce apoptosis in Ras-expressing keratinocytes and murine squamous cell carcinoma cells while avoiding cytotoxicity in untransformed keratinocytes. When administered to transgenic mice with HRasG12V-driven skin tumors, a single dose of silver nanoparticle conjugates followed by 415 nm LED irradiation at the tumor site caused a rapid and sustained reduction in tumor volume by 92.8%, recruited T cells to the tumor site, and acted as a potent immunomodulator by polarizing the cytokine balance toward Th1 both locally and systemically. In summary, our results demonstrate that spatiotemporal controlled miR-148b mimic delivery can promote tumor regression efficiently and safely.
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影响因子：
17.4
作者：
Gustafson HH;Holt-Casper D;Grainger DW;Ghandehari H
通讯作者：
Ghandehari H
影响因子：
14
作者：
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Zeng, Yi
影响因子：
3.4
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16.1
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Chen, Yunching;Gao, Dong-Yu;Huang, Leaf
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Huang, Leaf
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影响因子：
5.6
作者：
Chen NT;Cheng SH;Liu CP;Souris JS;Chen CT;Mou CY;Lo LW
通讯作者：
Lo LW