The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva.
The differences in bioaccumulation and effects between Se(IV) and Se(VI) in the topmouth gudgeon Pseudorasbora parva.
复制标题
Se(IV)和Se(VI)在顶嘴鱼体内的生物富集性和效应差异
DOI：
10.1038/s41598-018-32270-z
复制
发表时间：
2018-09-14
影响因子：
4.6
通讯作者：
Zhang Q
中科院分区：
文献类型：
--
作者：
Ma S;Zeng X;Chen H;Geng S;Yan L;Luo Y;Xie L;Zhang Q
硒(Se)可能在营养水平上对氧化应激具有保护作用，但饲料中Se浓度的升高已被揭示为受Se污染的湖泊中天然鱼类种群灭绝的主要罪魁祸首。虽然硒在水中主要以水中的亚硒酸盐(IV)和亚硒酸盐(VI)的形式存在，但在生物积累、影响(如氧化应激、抗氧化剂等)方面的差异。而在野生鱼类中，Se(IV)和Se(VI)之间的分子机制研究相对较少。在本研究中，将微小对虾暴露于水中的Se(10,200和1000 μg/L的Se(IV)或Se(VI))，并在第4，14和28天采样。评价了硒在不同组织中的生物累积、组织分布及后续效应。结果表明，与Se(VI)处理相比，Se(IV)处理显著提高了鳃和肠道中Se的含量，且呈浓度依赖性，暴露28d后，分别增加了173.3%和57.2%。此外，Se(IV)处理组的肌肉中也观察到了显著的Se积累。在Se(IV)处理中，Se暴露增加了脑和鳃中的MDA水平，但在Se(VI)处理中不那么明显。同时，暴露28d后，鳃中GST活性降低(至少56%)，而肌肉(~69%)和脑(~50%)中AChE活性升高。最重要的是，暴露28d后，暴露于最高试验浓度下的鳃(至少35%)和所有组织中的GSH水平显著下降。总体来看，Se(IV)导致青海对虾体内Se的积累速度快于Se(VI)，其产生的脂质过氧化作用与抗氧化剂的水平密切相关，尤其是GSH。我们的结果表明，在野外的淡水物种中，水性亚硒酸盐和亚硒酸盐的生态毒理效应不同。
Selenium (Se) might be protective against oxidative stress at nutritional levels, but elevated Se concentrations in the diet has been revealed as the main culprit for the extinction of natural fish populations in Se-contaminated lakes. Though Se predominate as waterborne selenite (IV) and selenate (VI) in the water, the differences in bioaccumulation, effects (e.g., oxidative stress, antioxidants etc.) and molecular mechanisms between Se(IV) and Se(VI) have been relatively understudied in wild fish. In this study, the P. parva were exposed to waterborne Se (10, 200 and 1000 μg/L of Se(IV) or Se(VI)) and sampled at 4, 14 and 28 days. Bioaccumulation, tissue distributions of Se and following effects in different tissues were evaluated. The results showed that the levels of Se in the gills and intestine were significantly elevated with a seemingly concentration-dependent pattern in the Se(IV) treatment, with respectively 173.3% and 57.2% increase after 28 days of exposure, relative to that of Se(VI) treatment. Additionally, significant accumulation of Se was also observed in the muscle of Se(IV) treated fish. Se exposure increased the MDA levels in the brain and gills in the Se(IV) treatment, but less apparent in the Se(VI) treatment. Meanwhile, Se exposure lowered (at least 56%) the activity of GST in the gills, but increased the activity of AChE in the muscle (~69%) and brain (~50%) after 28 d. Most importantly, after 28 d of exposure, Se exposure caused significant decrease in GSH levels in the gills (at least 35%) and in all tissues examined at the highest test concentration. In general, the results showed that Se(IV) led to faster accumulation of Se than Se(VI) in P. parva, and the resulted lipid peroxidation was closely related to the levels of antioxidants, especially GSH. Our results suggest that the ecotoxicological effects of waterborne selenite and selenate differ in this freshwater species in the field.
登录
查看更多内容
影响因子：
3.5
作者：
Kucukbay, F. Z.;Yazlak, H.;Sahin, K.
通讯作者：
Sahin, K.
影响因子：
2.9
作者：
Hamilton, SJ;Buhl, KJ
通讯作者：
Buhl, KJ
影响因子：
4.5
作者：
LEMLY, AD
通讯作者：
LEMLY, AD
影响因子：
4.1
作者：
Franz, Eric D.;Wiramanaden, Cheryl I. E.;Libert, Karsten
通讯作者：
Libert, Karsten
影响因子：
24.5
作者：
Hoensch, H;Morgenstern, I;Kirch, W
通讯作者：
Kirch, W