Interventional Efects of the Topical of "Sanse Powder" Essential Oils Nanoemulsion on Knee Osteoarthritis in Rats by Targeting the ERS/TXNIP/NLRP3 Signaling Axis.
Interventional Efects of the Topical of "Sanse Powder" Essential Oils Nanoemulsion on Knee Osteoarthritis in Rats by Targeting the ERS/TXNIP/NLRP3 Signaling Axis.
复制标题
以ERS/TXNIP/NLRP3信号轴为靶点的三色散纳米乳剂对大鼠膝骨性关节炎的干预作用
DOI：
10.3389/fphar.2021.739644
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发表时间：
2021
影响因子：
5.6
通讯作者：
Wang P
中科院分区：
文献类型：
--
作者：
Liu Z;Liao T;Yang N;Ding L;Li X;Wu P;Wang P
目的：我们近期的研究致力于从传统中医药中寻找治疗膝骨关节炎（KOA）的有效药物，并试图充分利用现代科学技术来揭示其背后的机制和靶点。滑膜炎症是KOA的主要病理特征之一，越来越多的研究者意识到早期干预滑膜炎症可能能够逆转疾病进展。传统天然产物与现代纳米技术的密切联系可能对提高抗滑膜炎疗效很重要。本研究的目的是探讨NEs-SP-EO抗滑膜炎的作用机制，其机制可能与ERS/TXNIP/NLRP 3信号轴有关。研究方法：采用GC-MS分析了“三色散”精油（SP-EO）和NEs-SP-EO的化学组成，制备了NEs-SP-EO，并通过纳米粒子跟踪分析、多分散指数（PDI）、zeta电位（ZP）、紫外-可见光谱和透射电子显微镜对其进行了表征。采用CCK-8法检测NEs-SP-EO对成纤维样滑膜细胞（FLS）的细胞活力，并通过LPS刺激炎症环境来探讨其体外治疗机制。在雄性SD大鼠上进行了NEs-SP-EO体内实验。结果：GC-MS分析表明，SP-EO中含有30个化合物，NES-SP-EO中鉴定出11个化合物。结果还表明，NEs-SP-EO具有合适的粒径、负电荷和稳定的体系。在体外和体内试验中，NEs-SP-EO通过减少ERS/TXNIP/NLRP 3信号轴的诱导以及调节IL-1β、IL-18的过度产生而产生抗滑膜炎功效。结论：三色散挥发油纳米乳具有治疗膝骨性关节炎滑膜炎的作用。此外，我们还初步探讨了可能与ERS/TXNIP/NLRP 3信号轴有关的抗炎机制。
Purpose: Our recent research is dedicated to finding effective drugs for the treatment of knee osteoarthritis (KOA) from traditional Chinese medicine and trying to make full use of modern science and technology to uncover the mechanisms and targets behind them. Synovial inflammation is one of the key pathological features of KOA, and a growing number of researchers realize that early intervention of synovial inflammation may be able to reverse disease progression. The close association of traditional natural products with modern nanotechnology may be important for improving the anti-synovitis efficacy. The purpose of our research was to explore the anti-synovitis mechanism of NEs-SP-EO that might be associated with the ERS/TXNIP/NLRP3 signalling axis. Methods: Chemical composition of “Sanse Powder” essential oil (SP-EO) and NEs-SP-EO were analyzed by GC-MS. NEs-SP-EO were prepared and characterized by nanoparticle tracking analysis, polydispersity index (PDI), zeta potential (ZP), ultraviolet-visible spectroscopy, and transmission electronic microscopy. The CCK8 assay for cell viability of NEs-SP-EO was performed on fibroblast-like synovial cells (FLSs) and the inflammatory environment was stimulated by LPS to explore the therapeutic mechanisms in vitro. Experiments of NEs-SP-EO in vivo were performed in male SD rats. Results: The GC-MS results showed that 30 compounds were present in SP-EO and 11 components of NEs-SP-EO were identified. The results also showed that the formulation of NEs-SP-EO exhibited suitable particle size, negative charge, and stable system. In vitro and vivo testing, NEs-SP-EO produced anti-synovitis efficacy by reduced the induction of the ERS/TXNIP/NLRP3 signaling axis as well as regulating the overproduction of IL-1β, IL-18. Conclusion: We have developed a new type of essential oil nanoemulsion from “Sanse Powder” and demonstrated that it can managing synovitis of KOA. Besides, we have initially explored the anti-inflammatory mechanism that may be related to the ERS/TXNIP/NLRP3 signaling axis.
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影响因子：
8
作者：
Gaspar de Toledo L;Dos Santos Ramos MA;Bento da Silva P;Rodero CF;de Sá Gomes V;Noronha da Silva A;Pavan FR;da Silva IC;Bombarda Oda F;Flumignan DL;Gonzaga Dos Santos A;Chorilli M;Gottardo de Almeida MT;Bauab TM
通讯作者：
Bauab TM
影响因子：
7
作者：
Bannuru, R. R.;Osani, M. C.;McAlindon, T. E.
通讯作者：
McAlindon, T. E.
影响因子：
5.2
作者：
Dosoky, Noura S.;Satyal, Prabodh;Setzer, William N.
通讯作者：
Setzer, William N.
影响因子：
1.8
作者：
Kotynska, Joanna;Figaszewski, Zbigniew A.
通讯作者：
Figaszewski, Zbigniew A.
DOI：
10.3390/molecules191220091
发表时间：
2014-12-01
期刊：
Molecules (Basel, Switzerland)
影响因子：
--
作者：
Priyadarsini KI
通讯作者：
Priyadarsini KI