Complete bacteriophage transfer in a bacterial endosymbiont (Wolbachia) determined by targeted genome capture.
Complete bacteriophage transfer in a bacterial endosymbiont (Wolbachia) determined by targeted genome capture.
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DOI：
10.1093/gbe/evr007
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发表时间：
2011
影响因子：
3.3
通讯作者：
Bordenstein SR
中科院分区：
文献类型：
--
作者：
Kent BN;Salichos L;Gibbons JG;Rokas A;Newton IL;Clark ME;Bordenstein SR
噬菌体通量可以导致自由生活细菌的大部分遗传多样性。细菌基因组进化的这一原则一般不延伸到专性胞内细菌，因为它们与其他微生物的接触减少，并且基因缺失的优势超过了基因转移。然而，最近的研究表明，同一宿主的细胞内联合感染可以促进专性细胞内细菌之间的移动元素的交换--通过这种方式，这些细菌可以部分缓解细胞内生活方式的还原力。为了测试噬菌体是以单基因的形式在联合感染之间转移还是以更大的区域进行转移，我们对寄生黄蜂的专性细胞内沃尔巴克氏菌菌株wVitB的基因组进行了测序，并将其与分歧的wVitA联合感染的前驱噬菌体序列进行了比较。我们首次应用了靶向序列捕获阵列来从真核、细菌和病毒DNA的异质混合物中特异性地捕获共生菌的DNA。该平铺阵列以98.3%的效率成功捕获了基因组。对基因组序列的检测揭示了迄今为止在两个专性细胞内联合感染之间最大的噬菌体和侧翼基因转移(52.2kb)。基于两株噬菌体序列99.9%的平均核苷酸同源性，在最近的进化过程中发生了移动元件的转移。除了发现共同感染专职细胞内细菌之间最近的大规模水平噬菌体转移外，我们还证明了“靶向基因组捕获”可以丰富目标DNA，以缓解从真核生物内的生物体中分离出难以培养或纯化的共生微生物的问题。
Bacteriophage flux can cause the majority of genetic diversity in free-living bacteria. This tenet of bacterial genome evolution generally does not extend to obligate intracellular bacteria owing to their reduced contact with other microbes and a predominance of gene deletion over gene transfer. However, recent studies suggest intracellular coinfections in the same host can facilitate exchange of mobile elements between obligate intracellular bacteria—a means by which these bacteria can partially mitigate the reductive forces of the intracellular lifestyle. To test whether bacteriophages transfer as single genes or larger regions between coinfections, we sequenced the genome of the obligate intracellular Wolbachia strain wVitB from the parasitic wasp Nasonia vitripennis and compared it against the prophage sequences of the divergent wVitA coinfection. We applied, for the first time, a targeted sequence capture array to specifically trap the symbiont's DNA from a heterogeneous mixture of eukaryotic, bacterial, and viral DNA. The tiled array successfully captured the genome with 98.3% efficiency. Examination of the genome sequence revealed the largest transfer of bacteriophage and flanking genes (52.2 kb) to date between two obligate intracellular coinfections. The mobile element transfer occurred in the recent evolutionary past based on the 99.9% average nucleotide identity of the phage sequences between the two strains. In addition to discovering an evolutionary recent and large-scale horizontal phage transfer between coinfecting obligate intracellular bacteria, we demonstrate that “targeted genome capture” can enrich target DNA to alleviate the problem of isolating symbiotic microbes that are difficult to culture or purify from the conglomerate of organisms inside eukaryotes.
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古老的内共生体内插入序列的强烈替代活性。
影响因子：
10.7
作者：
Cordaux, Richard;Pichon, Samuel;Ling, Alison;Perez, Philippe;Delaunay, Carine;Vavre, Fabrice;Bouchon, Didier;Greve, Pierre
通讯作者：
Greve, Pierre
影响因子：
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作者：
Baldridge, Gerald D.;Burkhardt, Nicole Y.;Munderloh, Ulrike G.
通讯作者：
Munderloh, Ulrike G.
影响因子：
5.8
作者：
Andersson, SGE;Alsmark, C;Tamas, I
通讯作者：
Tamas, I
影响因子：
10.7
作者：
Gavotte, Laurent;Henri, Helene;Vavre, Fabrice
通讯作者：
Vavre, Fabrice
影响因子：
5.8
作者：
Hsia, RC;Ohayon, H;Bavoil, PM
通讯作者：
Bavoil, PM