Intense transpositional activity of insertion sequences in an ancient obligate endosymbiont.
Intense transpositional activity of insertion sequences in an ancient obligate endosymbiont.
复制标题
古老的内共生体内插入序列的强烈替代活性。
DOI：
10.1093/molbev/msn134
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发表时间：
2008-09
影响因子：
10.7
通讯作者：
Greve, Pierre
中科院分区：
文献类型：
--
作者：
Cordaux, Richard;Pichon, Samuel;Ling, Alison;Perez, Philippe;Delaunay, Carine;Vavre, Fabrice;Bouchon, Didier;Greve, Pierre
关键词：
古代专性内共生体的流线型基因组通常缺乏转座因子，如插入序列（IS）。然而，地球上最丰富的细菌内共生体之一沃尔巴克氏体的基因组中充满了IS。这样一个悖论提出了一个问题，为什么在这个古老的内共生体的基因组中有这么多的IS。为了解决这个问题，我们调查了在unculturable沃尔巴克氏体的ISWpi1元素的进化动力学和历史的跟踪是易位活动。我们发现，1）ISWpi1是广泛的沃尔巴克氏体，存在于至少55%的40个采样菌株，2）ISWpi1拷贝表现出几乎相同的核苷酸序列内和之间的沃尔巴克氏体基因组，并拥有一个完整的转座酶基因，3）个别ISWpi1拷贝之间的差异插入沃尔巴克氏体基因组，和4）ISWpi1发生在沃尔巴克氏体基因组之间的可变拷贝数。总的来说，我们的结果为最近沃尔巴克氏体进化期间菌株之间强烈的ISWpi 1转座活动和频繁的ISWpi 1水平传播提供了令人信服的证据。因此，古专性内共生体的基因组可以携带高负荷的功能和转座活性转座因子。我们的研究结果还表明，沃尔巴克氏体基因组经历了多次和时间上不同的ISWpi1入侵在其进化历史。这种反复暴露于新的IS入侵可能解释，至少部分地，在沃尔巴克氏体内共生菌的基因组中发现的转座因子的密度异常高。
The streamlined genomes of ancient obligate endosymbionts generally lack transposable elements, such as insertion sequences (IS). Yet, the genome of Wolbachia, one of the most abundant bacterial endosymbionts on Earth, is littered with IS. Such a paradox raises the question as to why there are so many ISs in the genome of this ancient endosymbiont. To address this question, we investigated IS transpositional activity in the unculturable Wolbachia by tracking the evolutionary dynamics and history of ISWpi1 elements. We show that 1) ISWpi1 is widespread in Wolbachia, being present in at least 55% of the 40 sampled strains, 2) ISWpi1 copies exhibit virtually identical nucleotide sequences both within and among Wolbachia genomes and possess an intact transposase gene, 3) individual ISWpi1 copies are differentially inserted among Wolbachia genomes, and 4) ISWpi1 occurs at variable copy numbers among Wolbachia genomes. Collectively, our results provide compelling evidence for intense ISWpi1 transpositional activity and frequent ISWpi1 horizontal transmission among strains during recent Wolbachia evolution. Thus, the genomes of ancient obligate endosymbionts can carry high loads of functional and transpositionally active transposable elements. Our results also indicate that Wolbachia genomes have experienced multiple and temporally distinct ISWpi1 invasions during their evolutionary history. Such recurrent exposition to new IS invasions may explain, at least partly, the unusually high density of transposable elements found in the genomes of Wolbachia endosymbionts.
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影响因子：
12.3
作者：
Salzberg SL;Dunning Hotopp JC;Delcher AL;Pop M;Smith DR;Eisen MB;Nelson WC
通讯作者：
Nelson WC
DOI：
10.1098/rspb.1998.0402
发表时间：
1998-06-22
影响因子：
4.7
作者：
Bouchon, D;Rigaud, T;Juchault, P
通讯作者：
Juchault, P
影响因子：
10.7
作者：
Gavotte, Laurent;Henri, Helene;Vavre, Fabrice
通讯作者：
Vavre, Fabrice
影响因子：
3.8
作者：
Cordaux, R;Michel-Salzat, A;Bouchon, D
通讯作者：
Bouchon, D
影响因子：
3.5
作者：
Cordaux, Richard;Lee, Jungnam;Batzer, Mark A.
通讯作者：
Batzer, Mark A.