Discrimination of the Plasmodium mexicanum vectors Lutzomyia stewarti and Lutzomyia vexator by a PCR-RFLP assay and Wolbachia infection.
Discrimination of the Plasmodium mexicanum vectors Lutzomyia stewarti and Lutzomyia vexator by a PCR-RFLP assay and Wolbachia infection.
复制标题
DOI：
10.1111/j.1948-7134.2014.12092.x
复制
发表时间：
2014-06
期刊：
影响因子：
--
通讯作者：
Vardo-Zalik AM
中科院分区：
文献类型：
--
作者：
Hughes GL;Samuels SK;Shaikh K;Rasgon JL;Vardo-Zalik AM
Lutzomyia vexator（Coquillett 1907）和L. stewarti（Mangabeira Fo & Galindo 1944）是已报道的14种沙蝇（双翅目：蝇科）中的两种，栖息在北美（Young和Perkins 1984）。这两个物种在加州同域分布（Ayala 1973，Young and Perkins 1984），并且经常占据相同的啮齿动物洞穴作为休息场所。这两个物种都将蜥蜴类疟疾寄生虫墨西哥疟原虫传播给北方加州的蜥蜴（Ayala，1973年，Schall 2000年，Vardo-Zalik 2009年）。此外，这些苍蝇也被记录为其他爬行动物病原体的宿主，包括锥虫、血红蛋白和gregarines（Ayala 1973）。因此，了解这些媒介对于了解爬行动物疾病生态学非常重要。在纽约北部接近犬利什曼病爆发的地方发现了Lutzomyia vexator，也被认为是犬利什曼病的潜在载体（Ostfeldt et al.2004）。stewarti和L. vexator将有助于在这个系统中进行研究。目前，这些蝇通过形态学特征来识别，例如雌性受精囊形状或雄性上抱茎上刺的排列和数量（Young和Perkins 1984）。在捕获过程中，苍蝇很容易受到伤害，这使得实地形态识别耗时且经常存在问题。由于对这些捕获的蝇进行常规分子分析以获取寄生虫遗传信息，因此用于种属鉴定的分子分析是合乎逻辑的（Weng等人，2012年;明特等人，2013年）。本研究的目的是建立一种简单的分子生物学方法来鉴定这两种白蛉。鉴定基于线粒体COI基因的序列变异和细菌内共生体Wolbachia的存在/不存在。
Lutzomyia vexator (Coquillett 1907) and L. stewarti (Mangabeira Fo & Galindo 1944), are two of the fourteen reported species of sand flies (Diptera: Psychodidae) inhabiting North America (Young and Perkins 1984). These two species are found in sympatry in California (Ayala 1973, Young and Perkins 1984), and often occupy the same rodent burrows for resting sites. Both species transmit the saurian malaria parasite, Plasmodium mexicanum, to lizards in northern California (Ayala, 1973, Schall 2000, Vardo-Zalik 2009). Additionally, these flies have been documented as hosts for other reptilian pathogens, including trypanosomes, haemogregarines, and gregarines (Ayala 1973). Therefore, knowledge of these vectors is important for understanding reptilian disease ecology. Lutzomyia vexator has also been implicated as a potential vector for canine leishmaniasis after being found in upstate New York proximal to a canine leishmanisis outbreak (Ostfeldt et al. 2004).As the only known vectors for P. mexicanum, one of the most well-studied saurian malaria parasites, development of a reliable molecular identification method to differentiate L. stewarti and L. vexator would facilitate studies in this system. Currently, these flies are identified by morphological traits, such as the spermatheca shape for females or the arrangement and number of spines on the upper claspers for males (Young and Perkins 1984). During capture, flies are easily damaged, which makes morphological identification in the field time-consuming and often problematic. As molecular analyses are routinely performed on these captured flies for parasite genetic information, molecular analysis for species identification is logical (Weng et al. 2012, Minter et al. 2013). The purpose of this study was to develop a simple molecular method for species identification for these two sand fly species. Identifications were based on sequence variation in the mitochondrial COI gene and presence/absence of the bacterial endosymbiont Wolbachia.
登录
查看更多内容
影响因子：
2.1
作者：
CHANIOTIS, BN;ANDERSON, JR
通讯作者：
ANDERSON, JR
影响因子：
2.1
作者：
Ostfeld, RS;Roy, P;Rowton, ED
通讯作者：
Rowton, ED
影响因子：
2.4
作者：
Schall, JJ
通讯作者：
Schall, JJ
影响因子：
2.1
作者：
Zele, F.;Nicot, A.;Rivero, A.
通讯作者：
Rivero, A.
影响因子：
2.1
作者：
Ono, M;Braig, HR;O'Neill, SL
通讯作者：
O'Neill, SL