The Clinical Use of Human Culture-Expanded Autologous Bone Marrow Mesenchymal Stem Cells Transplanted on Platelet-Rich Fibrin Glue in the Treatment of Articular Cartilage Defects: A Pilot Study and Preliminary Results.
The Clinical Use of Human Culture-Expanded Autologous Bone Marrow Mesenchymal Stem Cells Transplanted on Platelet-Rich Fibrin Glue in the Treatment of Articular Cartilage Defects: A Pilot Study and Preliminary Results.
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DOI：
10.1177/1947603510366027
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发表时间：
2010-10
期刊：
影响因子：
2.8
通讯作者：
Abdel Aziz MT
中科院分区：
文献类型：
--
作者：
Haleem AM;Singergy AA;Sabry D;Atta HM;Rashed LA;Chu CR;El Shewy MT;Azzam A;Abdel Aziz MT
验证富血小板纤维蛋白胶（PR-FG）可在临床上用作支架输送自体培养扩增的骨髓间充质干细胞（BM-MSCs）用于软骨修复的假设，并报告MSCs PR-FG植入后1年的临床结果。将自体骨髓间充质干细胞培养扩增后，置于PR-FG上，移植于5例患者股骨髁全层软骨缺损处，并覆盖自体骨膜瓣。在6个月和12个月时，通过Lysholm和膝关节特殊外科医院（RHSSK）评分对患者进行临床评价，并在相同时间点通过X线和磁共振成像（MRI）进行影像学评价。12个月后，使用国际腕关节修复学会（ICRS）关节镜评分对2例患者的修复组织进行了关节镜评分。病例系列;证据等级4。所有患者的症状在12个月的随访期内得到改善。所有患者的Lysholm和RHSSK评分在术后6个月和12个月均显示出统计学显著改善（P < 0.05）。术后6个月和12个月临床评分之间无统计学显著差异（P = 0.18）。同意接受关节镜检查的2例患者的ICRS关节镜评分分别为8/12和11/12（接近正常）。术后12个月，3例患者的MRI显示缺损完全填充，表面与天然软骨完全一致，而2例患者显示不完全一致。自体骨髓间充质干细胞移植于PR-FG作为细胞支架可能是促进人类膝关节软骨缺损修复的有效途径。
To test the hypothesis that platelet-rich fibrin glue (PR-FG) can be used clinically as a scaffold to deliver autologous culture-expanded bone marrow mesenchymal stem cells (BM-MSCs) for cartilage repair and to report clinical results 1 y after implantation of MSCs PR-FG. Autologous BM-MSCs were culture expanded, placed on PR-FG intraoperatively, and then transplanted into 5 full-thickness cartilage defects of femoral condyles of 5 patients and covered with an autologous periosteal flap. Patients were evaluated clinically at 6 and 12 mo by the Lysholm and Revised Hospital for Special Surgery Knee (RHSSK) scores and radiographically by x-rays and magnetic resonance imaging (MRI) at the same time points. Repair tissue in 2 patients was rated arthroscopically after 12 mo using the International Cartilage Repair Society (ICRS) Arthroscopic Score. Case series; level of evidence 4. All patients’ symptoms improved over the follow-up period of 12 mo. Average Lysholm and RHSSK scores for all patients showed statistically significant improvement at 6 and 12 mo postoperatively (P < 0.05). There was no statistically significant difference between the 6 and 12 mo postoperative clinical scores (P = 0.18). ICRS arthroscopic scores were 8/12 and 11/12 (nearly normal) for the 2 patients who consented to arthroscopy. MRI of 3 patients at 12 mo postoperatively revealed complete defect fill and complete surface congruity with native cartilage, whereas that of 2 patients showed incomplete congruity. Autologous BM-MSC transplantation on PR-FG as a cell scaffold may be an effective approach to promote the repair of articular cartilage defects of the knee in human patients.
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影响因子：
2.4
作者：
Kotobuki, N;Hirose, M;Ohgushi, H
通讯作者：
Ohgushi, H
影响因子：
2.8
作者：
Aziz, M. T. Abdel;Atta, H. M.;Hasan, N. M.
通讯作者：
Hasan, N. M.
DOI：
10.1002/jbm.820290915
发表时间：
1995-09-01
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
CHU, CR;COUTTS, RD;AMIEL, D
通讯作者：
AMIEL, D
影响因子：
2.2
作者：
Nakahara, M;Takagi, M;Yoshida, T
通讯作者：
Yoshida, T
影响因子：
2.7
作者：
O'Neill, EM;Zalewski, WM;Valeri, CR
通讯作者：
Valeri, CR