Multiple drug resistance and biocide resistance in Escherichia coli environmental isolates from hospital and household settings.
Multiple drug resistance and biocide resistance in Escherichia coli environmental isolates from hospital and household settings.
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DOI：
10.1186/s13756-018-0339-8
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发表时间：
2018
影响因子：
5.5
通讯作者：
Haddadin RN
中科院分区：
文献类型：
--
作者：
Ghanem B;Haddadin RN
由于大量使用杀生剂导致严重感染，医院环境中的大肠埃希菌的耐药性可能会增加。本研究对医院和家庭环境中分离的大肠埃希菌的耐药性进行了评估和比较。此外，还探讨了杀菌剂最低抑菌浓度(MIC)与多重耐药(MDR)的关系。环境样本是从约旦安曼的不同家庭和医院收集的。对分离到的菌株进行了表型鉴定和聚合酶链式反应。对分离的菌株进行药敏试验和MIC试验。同时对blaCTX-M组1进行筛查。在分离的21株大肠埃希菌中，47.6%为耐多药菌，67.9%为产超广谱β-内酰胺酶(ESBL)。这些超广谱β-内酰胺酶分离株在家庭和医院环境中的发生率是相似的(P >； 0.05)。这些杀生剂对所有菌株的最低抑菌浓度均远低于使用浓度。此外，家庭分离株和医院分离株对杀生剂的MIC值相当(P >； 005)。未发现多药耐药与杀菌剂最低抑菌浓度之间存在关联(P &> )。大多数ESBL分离株携带blaCTX-M1。杀生剂在医院的广泛使用与MDR无关，也不影响杀生剂对大肠杆菌的MIC。
Antibiotic resistance of environmental Escherichia coli in hospitals could be increased due to extensive use of biocides resulting in serious infections. In this study, the prevalence of antibiotic resistance of environmental isolates of E. coli from hospitals and household settings were evaluated and compared. In addition, the association between biocide minimum inhibitory concentration (MIC) and multiple drug resistance (MDR) was investigated. Environmental samples were collected from different homes and hospitals in Amman, Jordan. The isolates were identified phenotypically and by PCR. Antibiotic susceptibility tests and MIC of selected biocides were performed on the isolates. Screening for blaCTX-M group 1 was also performed. Of 21 E. coli strains isolated, 47.6% were MDR and 67.9% were phenotypically identified as extended spectrum beta-lactamase (ESBL) producers. The occurrence of these ESBL isolates was comparable between household and hospital settings (P > 0.05). The MIC values of the biocides tested against all isolates were well below the in-use concentration of biocides. Moreover, the MICs of biocides were comparable between isolates from households and those from hospitals (P > 0.05). No association was found between MDR and biocide MIC (P > 0.05). Most of ESBL isolates harboured blaCTX-M 1. The extensive use of biocides in hospitals is not associated with MDR nor does it affect the MIC of biocides against E.coli.
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DOI：
10.4103/0970-0218.137152
发表时间：
2014-07-01
期刊：
Indian journal of community medicine : official publication of Indian Association of Preventive & Social Medicine
影响因子：
--
作者：
Poonia, Shubra;Singh, T Shantikumar;Tsering, Dechen C
通讯作者：
Tsering, Dechen C
影响因子：
4
作者：
Tsen, HY;Lin, CK;Chi, WR
通讯作者：
Chi, WR
影响因子：
5.2
作者：
McBain, AJ;Ledder, RG;Gilbert, P
通讯作者：
Gilbert, P
影响因子：
--
作者：
Mirzaee, Mohsen;Owlia, Parviz;Mansouri, Sadegh
通讯作者：
Mansouri, Sadegh
影响因子：
4
作者：
Ledder, R. G.;Gilbert, P.;McBain, A. J.
通讯作者：
McBain, A. J.